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APOA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9606923

Description: ApoAI (Apolipoprotein A1) is a major component of high density lipoprotein (HDL, the "good cholesterol") in plasma. It is produced in the liver and small intestine. ApoA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) that is… More details

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Product Overview

Online Datasheet in PDF
Product Name
APOA1, Polyclonal Antibody
Full Product Name
APOA1 Antibody
Product Gene Name
anti-APOA1 antibody
Product Synonym Names
Apo-AI; ApoA I; ApoA-I; APOA1; APOA1_HUMAN; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; Brp14; Ltw1; Lvtw1; Sep1; Sep2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
APOA1 antibody detects endogenous levels of total APOA1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
APOA1 peptide (MBS9625640) is used for blocking the activity of APOA1 antibody (MBS9606923)
Molecular Weight
Observed: 31 kDa
Predicted: 31 kDa
3D Structure
ModBase 3D Structure for P02647
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human APOA1
Subcellular Location
Secreted.
Tissue Specificity
Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-APOA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-APOA1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-APOA1 antibody

Western blot analysis of brain using APOA1 antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-APOA1 Polyclonal Antibody – Cat# MBS9606923

Immunohistochemistry (IHC) of anti-APOA1 antibody

MBS9606923 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-APOA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9606923

Western Blot (WB) of anti-APOA1 antibody

Western blot analysis of APOA1 expression in various lysates
Rabbit Anti-APOA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606923

Western Blot (WB) of anti-APOA1 antibody

Western blot analysis of APOA1 expression in Mouse brain lysate
Rabbit Anti-APOA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606923

Other Notes

Small volumes of anti-APOA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOA1 purchase

MBS9606923 is an antibody targeting APOA1. Also known as APOA1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APOA1. It may not necessarily be applicable to this product.

NCBI GI #
4557321
NCBI GeneID
335
NCBI Accession #
NP_000030.1
UniProt Primary Accession #
P02647
UniProt Secondary Accession #
Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866
UniProt Related Accession #
P02647
GenBank Nucleotide #
NM_000039.2
NCBI Official Full Name
apolipoprotein A-I isoform 1 preproprotein
NCBI Official Synonym Full Names
apolipoprotein A1
NCBI Official Symbol
APOA1
NCBI Official Synonym Symbols
apo(a)
UniProt Protein Name
Apolipoprotein A-I
UniProt Synonym Protein Names
Apolipoprotein A1
Protein Family
Apolipoprotein
UniProt Gene Name
APOA1
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I
NCBI Protein Information
apolipoprotein A-I

NCBI Summary for APOA1

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
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UniProt Comments for APOA1

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Research Articles on APOA1

1. An increased apo B/apo A1 ratio was independently associated with all-cause mortality and cardiovascular events in peritoneal dialysis patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APOA1 antibody

 Reference Product  PubMed Publications
 APOB antibody  >480 publications with APOA1 and APOB
 ABCA1 antibody  >129 publications with APOA1 and ABCA1
 APOC3 antibody  >69 publications with APOA1 and APOC3
 CETP antibody  >59 publications with APOA1 and CETP
 APOA2 antibody  >40 publications with APOA1 and APOA2
 LCAT antibody  >39 publications with APOA1 and LCAT
 ALB antibody  >8 publications with APOA1 and ALB
 TTR antibody  >7 publications with APOA1 and TTR
 FGA antibody  >2 publications with APOA1 and FGA

Pathways associated with anti-APOA1 antibody

 Products by Pathway  Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 ABCA Transporters In Lipid Homeostasis Pathway antibodies  ABCA Transporters In Lipid Homeostasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram

Diseases associated with anti-APOA1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >440 publications with APOA1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >269 publications with APOA1 and Atherosclerosis
 Dyslipidemias Antibodies  >182 publications with APOA1 and Dyslipidemias
 Hyperlipidemias Antibodies  >125 publications with APOA1 and Hyperlipidemias
 Inflammation Antibodies  >107 publications with APOA1 and Inflammation
 Insulin Resistance Antibodies  >97 publications with APOA1 and Insulin Resistance
 Hypertension Antibodies  >76 publications with APOA1 and Hypertension
 Hypercholesterolemia Antibodies  >64 publications with APOA1 and Hypercholesterolemia
 Hypertriglyceridemia Antibodies  >58 publications with APOA1 and Hypertriglyceridemia
 Liver Diseases Antibodies  >50 publications with APOA1 and Liver Diseases

Organs/Tissues associated with anti-APOA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >782 publications with APOA1 and Blood
 Liver Antibodies  >179 publications with APOA1 and Liver
 Heart Antibodies  >157 publications with APOA1 and Heart
 Brain Antibodies  >33 publications with APOA1 and Brain
 Muscle Antibodies  >33 publications with APOA1 and Muscle
 Lung Antibodies  >21 publications with APOA1 and Lung
 Ovary Antibodies  >15 publications with APOA1 and Ovary
 Intestine Antibodies  >15 publications with APOA1 and Intestine
 Pancreas Antibodies  >7 publications with APOA1 and Pancreas
 Embryonic Tissue Antibodies  >4 publications with APOA1 and Embryonic Tissue
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