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GAD65 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244350

MBS244350 is an antibody targeting GAD65. Also known as Goat Polyclonal to Human GAD65. Reactive in Gibbon, Gorilla, Human, Monkey, MousePredicted Reactivity: Rat, Bat, Hamster, Horse (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Overview

Online Datasheet in PDF
Product Name
GAD65, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human GAD65
Product Gene Name
anti-GAD65 antibody
Product Synonym Gene Name
GAD2
Product Synonym Names
Anti-GAD65 Antibody (Internal) IHC-plus; GAD2; GAD-65; Glutamate decarboxylase 2; GAD65; Human GAD65
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Monkey, Mouse
Predicted Reactivity: Rat, Bat, Hamster, Horse (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human GAD2 / GAD65. This antibody is expected to recognise both reported variants.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
65,411 Da
OMIM
138275
3D Structure
ModBase 3D Structure for Q05329
Target Species
Human
Immunogen Description
Synthetic peptide C-TLEDNEERMSRLSK from an internal region of human GAD2 / GAD65 (NP_000809.1; NP_001127838.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant (100%).
Immunogen Type
Synthetic peptide
Immunogen
GAD65 antibody was raised against synthetic peptide C-TLEDNEERMSRLSK from an internal region of human GAD2 / GAD65 (NP_000809.1; NP_001127838.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant (100%); Rat, Hamster, Panda, Bat, Horse (93%); Dog, Rabbit (86%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-GAD65 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GAD65 antibody
ELISA (1:32000), IHC-P (2.5 ug/ml), WB (1:32000, 0.1 - 0.3 ug/ml)

Immunohistochemistry (IHC) of anti-GAD65 antibody

Anti-GAD2 / GAD65 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-GAD65 Polyclonal Antibody – Cat# MBS244350

Immunohistochemistry (IHC) of anti-GAD65 antibody

Anti-GAD2 / GAD65 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-GAD65 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS244350

Western Blot (WB) of anti-GAD65 antibody

Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GAD65 Polyclonal Antibody, Western Blot (WB) – Cat# MBS244350

Other Notes

Small volumes of anti-GAD65 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD65. It may not necessarily be applicable to this product.

NCBI GI #
4503875
NCBI GeneID
2572
NCBI Accession #
NP_000809.1
UniProt Primary Accession #
Q05329
UniProt Secondary Accession #
Q9UD87
UniProt Related Accession #
Q05329
GenBank Nucleotide #
NM_000818.2
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
NCBI Official Symbol
GAD2
NCBI Official Synonym Symbols
GAD65
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
UniProt Gene Name
GAD2
UniProt Synonym Gene Names
GAD65; GAD-65
UniProt Entry Name
DCE2_HUMAN
NCBI Protein Information
glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform

NCBI Summary for GAD65

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
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UniProt Comments for GAD65

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Other Amino Acids Metabolism - beta-alanine; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
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Research Articles on GAD65

1. GAD2 SNPs significantly associate with early-onset obsessive compulsive disorder.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAD65 antibody

 Reference Product  PubMed Publications
 GAD1 antibody  >50 publications with GAD65 and GAD1
 GLUL antibody  >2 publications with GAD65 and GLUL
 GLS antibody  >1 publications with GAD65 and GLS
 INS antibody  >1 publications with GAD65 and INS
 SLC32A1 antibody  >1 publications with GAD65 and SLC32A1
 ABAT antibody  >1 publications with GAD65 and ABAT

Pathways associated with anti-GAD65 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD65 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >20 publications with GAD65 and Mental Disorders
 Nervous System Diseases Antibodies  >12 publications with GAD65 and Nervous System Diseases
 Brain Diseases Antibodies  >10 publications with GAD65 and Brain Diseases
 Schizophrenia Antibodies  >10 publications with GAD65 and Schizophrenia
 Epilepsy Antibodies  >6 publications with GAD65 and Epilepsy
 Seizures Antibodies  >6 publications with GAD65 and Seizures
 Depressive Disorder Antibodies  >4 publications with GAD65 and Depressive Disorder
 Inflammation Antibodies  >4 publications with GAD65 and Inflammation
 Movement Disorders Antibodies  >2 publications with GAD65 and Movement Disorders
 Substance Withdrawal Syndrome Antibodies  >2 publications with GAD65 and Substance Withdrawal Syndrome

Organs/Tissues associated with anti-GAD65 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >37 publications with GAD65 and Brain
 Pancreas Antibodies  >3 publications with GAD65 and Pancreas
 Uncharacterized Tissue Antibodies  >1 publications with GAD65 and Uncharacterized Tissue
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