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SLC39A14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244467

MBS244467 is an antibody targeting SLC39A14. Also known as Rabbit Polyclonal to Human SLC39A14 / ZIP14. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLC39A14 / ZIP14, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SLC39A14 / ZIP14
Product Gene Name
anti-SLC39A14 antibody
Product Synonym Gene Name
ZIP14
Product Synonym Names
Anti-SLC39A14 / ZIP14 Antibody (Internal) IHC-plus; SLC39A14; KIAA0062; LZT-Hs4; NET34; Zrt-; Irt-like protein 14; Zinc transporter ZIP14; ZIP-14; ZIP14; Zrt- and Irt-like protein 14; Cig19; Human SLC39A14; ZIP14
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
Human SLC39A14. At least three isoforms of ZIP14 are known to exist; this antibody will detect both isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.
Preparation and Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
OMIM
608736
3D Structure
ModBase 3D Structure for Q15043
Immunogen Type
Synthetic peptide
Immunogen Species
SLC39A14 / ZIP14 antibody was raised against Human
Immunogen
SLC39A14 / ZIP14 antibody was raised against a 16 amino acid peptide near the center of human ZIP14
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-SLC39A14 antibody

Family: Transporter; Subfamily: Zinc-iron transporter
Applications Tested/Suitable for anti-SLC39A14 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
Application Notes for anti-SLC39A14 antibody
IHC - Paraffin (5 µg/ml)
Western blot (1 - 2 µg/ml)
Optimal dilution to be determined by the researcher.
ZIP14 antibody can be used for detection of ZIP14 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 54 kDa Observed: 52 kDa

Immunohistochemistry (IHC) of anti-SLC39A14 antibody

Anti-SLC39A14 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SLC39A14 Polyclonal Antibody – Cat# MBS244467

Immunohistochemistry (IHC) of anti-SLC39A14 antibody

Anti-SLC39A14 antibody IHC of human colon, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SLC39A14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS244467

Immunofluorescence (IF) of anti-SLC39A14 antibody

Immunofluorescence of ZIP14 in Mouse Liver cells with ZIP14 antibody at 20 ug/ml.
Rabbit Anti-SLC39A14 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS244467

Western Blot (WB) of anti-SLC39A14 antibody

Western blot analysis of ZIP14 in human spleen tissue lysate with ZIP14 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-SLC39A14 Polyclonal Antibody, Western Blot (WB) – Cat# MBS244467

Other Notes

Small volumes of anti-SLC39A14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC39A14. It may not necessarily be applicable to this product.

NCBI GI #
190360568
NCBI GeneID
23516
NCBI Accession #
NP_001121903.1
UniProt Primary Accession #
Q15043
UniProt Secondary Accession #
Q6ZME8; Q96BB3; A6NH98; B4DIW3; B6EU88; D3DSR4
UniProt Related Accession #
Q15043
GenBank Nucleotide #
NM_001128431.2
NCBI Official Full Name
zinc transporter ZIP14 isoform a
NCBI Official Synonym Full Names
solute carrier family 39 (zinc transporter), member 14
NCBI Official Symbol
SLC39A14
NCBI Official Synonym Symbols
NET34; ZIP14; cig19; LZT-Hs4
UniProt Protein Name
Zinc transporter ZIP14
UniProt Synonym Protein Names
LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; Solute carrier family 39 member 14; Zrt- and Irt-like protein 14; ZIP-14
Protein Family
Zinc transporter
UniProt Gene Name
SLC39A14
UniProt Synonym Gene Names
KIAA0062; ZIP14; LZT-Hs4; ZIP-14
UniProt Entry Name
S39AE_HUMAN
NCBI Protein Information
zinc transporter ZIP14; ZIP-14; Zrt-, Irt-like protein 14; zrt- and Irt-like protein 14; solute carrier family 39 member 14; LIV-1 subfamily of ZIP zinc transporter 4; solute carrier family 39 (metal ion transporter), member 14

NCBI Summary for SLC39A14

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003 [PubMed 12659941]).[supplied by OMIM, Mar 2008]
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UniProt Comments for SLC39A14

SLC39A14: May mediate cellular uptake of nontransferrin-bound iron. Broad-scope metal ion transporter with a preference for zinc uptake. Belongs to the ZIP transporter (TC 2.A.5) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; lamellipodium; integral to membrane; plasma membrane

Molecular Function: zinc ion transmembrane transporter activity; ferrous iron transmembrane transporter activity

Biological Process: cellular zinc ion homeostasis; ferrous iron transport; transmembrane transport
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Research Articles on SLC39A14

1. Polymorphisms in SLC39A14 and SLC39A8 seemed to affect blood cadmium concentrations, for SLC39A14 this effect may occur via differential gene expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC39A14 antibody

 Reference Product  PubMed Publications
 SLC30A4 antibody  >3 publications with SLC39A14 and SLC30A4
 SLC30A1 antibody  >2 publications with SLC39A14 and SLC30A1
 MET antibody  >2 publications with SLC39A14 and MET
 SLC30A5 antibody  >1 publications with SLC39A14 and SLC30A5
 HFE antibody  >1 publications with SLC39A14 and HFE
 IL6 antibody  >1 publications with SLC39A14 and IL6

Pathways associated with anti-SLC39A14 antibody

 Products by Pathway  Pathway Diagram
 Metal Ion SLC Transporters Pathway antibodies  Metal Ion SLC Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram
 Zinc Influx Into Cells By The SLC39 Gene Family Pathway antibodies  Zinc Influx Into Cells By The SLC39 Gene Family Pathway Diagram
 Zinc Transporters Pathway antibodies  Zinc Transporters Pathway Diagram

Diseases associated with anti-SLC39A14 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with SLC39A14 and Inflammation
 Liver Diseases Antibodies  >4 publications with SLC39A14 and Liver Diseases
 Necrosis Antibodies  >3 publications with SLC39A14 and Necrosis
 Liver Neoplasms Antibodies  >2 publications with SLC39A14 and Liver Neoplasms
 Fibrosis Antibodies  >2 publications with SLC39A14 and Fibrosis
 Carcinoma, Hepatocellular Antibodies  >2 publications with SLC39A14 and Carcinoma, Hepatocellular
 Drug-Induced Liver Injury Antibodies  >2 publications with SLC39A14 and Drug-Induced Liver Injury
 Acute Kidney Injury Antibodies  >1 publications with SLC39A14 and Acute Kidney Injury
 Drug-Related Side Effects and Adverse Reactions Antibodies  >1 publications with SLC39A14 and Drug-Related Side Effects and Adverse Reactions
 Disease Models, Animal Antibodies  >1 publications with SLC39A14 and Disease Models, Animal

Organs/Tissues associated with anti-SLC39A14 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >19 publications with SLC39A14 and Liver
 Lung Antibodies  >9 publications with SLC39A14 and Lung
 Kidney Antibodies  >8 publications with SLC39A14 and Kidney
 Intestine Antibodies  >6 publications with SLC39A14 and Intestine
 Blood Antibodies  >4 publications with SLC39A14 and Blood
 Heart Antibodies  >4 publications with SLC39A14 and Heart
 Vascular Antibodies  >3 publications with SLC39A14 and Vascular
 Pancreas Antibodies  >3 publications with SLC39A14 and Pancreas
 Muscle Antibodies  >2 publications with SLC39A14 and Muscle
 Adipose Tissue Antibodies  >2 publications with SLC39A14 and Adipose Tissue
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