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ABCC4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421051

MBS421051 is an antibody targeting ABCC4. Also known as Goat anti-ABCC4/MRP4 Antibody. Reactive in Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ABCC4/MRP4, Polyclonal Antibody
Full Product Name
Goat anti-ABCC4/MRP4 Antibody
Product Gene Name
anti-ABCC4 antibody
Product Synonym Names
Goat Anti-ABCC4 (aa70-82) Antibody; ABCC4; MRP4; MOATB; MOAT-B; EST170205; ATP-binding cassette, sub-family C (CFTR/MRP), member 4; canalicular multispecific organic anion transporter (ABC superfamily); Multidrug Resistance Protein 4; RP11-74A12.1; ATP-binding cassette, sub-family C, member; ABCC4 antibody; MRP4 antibody; MOATB antibody; MOAT-B antibody; EST170205 antibody; ATP-binding cassette; sub-family C (CFTR/MRP); member 4 antibody; canalicular multispecific organic anion transporter (ABC superfamily) antibody; Multidrug Resistance Protein 4 antibody; RP11-74A12.1 antibody; ATP-binding cassette; sub-family C; member 4 antibody; bA464I2.1 (ATP-binding cassette; sub-family C (CFTR/MRP); member 4) antibody; canalicular multispecific organic anion transporter (ABC superfamily) antibody; multidrug resistance-associated protein 4; multispecific organic anion transporter B antibody; ABCC4 / MRP4
Immunogen Sequence
CNGQPSTLTI FETAL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ABCC4 / MRP4 peptide (MBS428478) is used for blocking the activity of ABCC4/MRP4 antibody (MBS421051)
Molecular Weight
88,138 Da
OMIM
605250
Immunogen
Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ABCC4 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ABCC4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data obtained from a previous batch and kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence: This antibody (from a previous batch) has been successfully used in ICC/IF for human primary hepatocytes as described in the following paper: Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G126-35, PMID: 20395535.

Immunohistochemistry (IHC) of anti-ABCC4 antibody

(4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-ABCC4 Polyclonal Antibody – Cat# MBS421051

Other Notes

Small volumes of anti-ABCC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCC4. It may not necessarily be applicable to this product.

NCBI GI #
157502201
NCBI GeneID
10257
NCBI Accession #
NP_005836.2
UniProt Secondary Accession #
Q8IVZ4; Q8IZN6; Q8NEW8; Q9Y6J2; A9Z1Z7; B7Z3Q7
UniProt Related Accession #
O15439
GenBank Nucleotide #
NM_005845.4
NCBI Official Full Name
multidrug resistance-associated protein 4 isoform 1
NCBI Official Synonym Full Names
ATP binding cassette subfamily C member 4
NCBI Official Symbol
ABCC4
NCBI Official Synonym Symbols
MRP4; MOATB; MOAT-B
UniProt Protein Name
Multidrug resistance-associated protein 4
UniProt Synonym Protein Names
ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B; MOAT-B
Protein Family
ABC transporter C family
UniProt Gene Name
ABCC4
UniProt Synonym Gene Names
MRP4; MOAT-B
UniProt Entry Name
MRP4_HUMAN
NCBI Protein Information
multidrug resistance-associated protein 4

NCBI Summary for ABCC4

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
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UniProt Comments for ABCC4

ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Transporter; Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: basolateral plasma membrane; integral to membrane; membrane; plasma membrane; platelet dense granule membrane

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; anion transmembrane-transporting ATPase activity; ATP binding; ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism

Biological Process: cilium biogenesis; platelet degranulation; prostaglandin secretion; transmembrane transport
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Research Articles on ABCC4

1. MRP4 genotyping may be useful for personalizing the therapeutic dose of 6-mercaptopurine during the acute lymphoblastic leukemia maintenance therapy in Japanese

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ABCC4 antibody

 Reference Product  PubMed Publications
 SLC22A6 antibody  >12 publications with ABCC4 and SLC22A6
 SLC22A8 antibody  >10 publications with ABCC4 and SLC22A8
 SLCO1B1 antibody  >8 publications with ABCC4 and SLCO1B1
 SLCO1A2 antibody  >5 publications with ABCC4 and SLCO1A2
 SNX27 antibody  >2 publications with ABCC4 and SNX27
 ABCG4 antibody  >1 publications with ABCC4 and ABCG4

Pathways associated with anti-ABCC4 antibody

 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Drug Induction Of Bile Acid Pathway antibodies  Drug Induction Of Bile Acid Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram

Diseases associated with anti-ABCC4 antibody

 Disease Name  Pubmed Publications
 Cholestasis Antibodies  >24 publications with ABCC4 and Cholestasis
 Inflammation Antibodies  >23 publications with ABCC4 and Inflammation
 Liver Diseases Antibodies  >23 publications with ABCC4 and Liver Diseases
 Disease Models, Animal Antibodies  >16 publications with ABCC4 and Disease Models, Animal
 Fibrosis Antibodies  >13 publications with ABCC4 and Fibrosis
 Brain Diseases Antibodies  >12 publications with ABCC4 and Brain Diseases
 Colorectal Neoplasms Antibodies  >12 publications with ABCC4 and Colorectal Neoplasms
 Kidney Diseases Antibodies  >11 publications with ABCC4 and Kidney Diseases
 Necrosis Antibodies  >11 publications with ABCC4 and Necrosis
 Drug-Induced Liver Injury Antibodies  >10 publications with ABCC4 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-ABCC4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >125 publications with ABCC4 and Blood
 Liver Antibodies  >89 publications with ABCC4 and Liver
 Kidney Antibodies  >84 publications with ABCC4 and Kidney
 Brain Antibodies  >52 publications with ABCC4 and Brain
 Vascular Antibodies  >26 publications with ABCC4 and Vascular
 Lung Antibodies  >25 publications with ABCC4 and Lung
 Intestine Antibodies  >21 publications with ABCC4 and Intestine
 Muscle Antibodies  >19 publications with ABCC4 and Muscle
 Eye Antibodies  >11 publications with ABCC4 and Eye
 Prostate Antibodies  >11 publications with ABCC4 and Prostate
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