Mouse Anti-NKIRAS1 Monoclonal Antibody – Cat# MBS245168

Anti-NKIRAS1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NKIRAS1 Antibody – Mouse Monoclonal

Name: NKIRAS1 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS245168
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245168

MBS245168 is an antibody targeting NKIRAS1. Also known as Mouse Monoclonal [clone 184C278] (IgG1) to Human NKIRAS1. Reactive in Gibbon, Dog, Gorilla, Goat, Hamster, Horse, Human, Monkey, Mouse, Rabbit, RatPredicted Reactivity: Bovine, Pig, Chicken (at least… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NKIRAS1, Monoclonal Antibody

Full Product Name

Mouse Monoclonal [clone 184C278] (IgG1) to Human NKIRAS1

Product Gene Name

anti-NKIRAS1 antibody

Product Synonym Names

Anti-NKIRAS1 Antibody (aa96-113, clone 184C278) IHC-plus; NKIRAS1; Kappa B-Ras protein 1; Kappa B-ras 1; KappaB-Ras1; KBRAS1; Human NKIRAS1

Clonality

Monoclonal

Isotype

IgG1

Clone Number

184C278

Host

Mouse

Species Reactivity

Gibbon, Dog, Gorilla, Goat, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat
Predicted Reactivity: Bovine, Pig, Chicken (at least 90% immunogen sequence identity)

Form/Format

PBS, 0.02% sodium azide.

Purity/Purification

Protein G Purified

Concentration

0.5 mg/ml (lot specific)

Specificity

A synthetic peptide containing amino acid residues 96-113 of human kB-Ras1.

Preparation and Storage

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Molecular Weight

21,643 Da

OMIM

604496

3D Structure

ModBase 3D Structure for Q9NYS0

Target Species

Human

Immunogen Description

A synthetic peptide containing amino acid residues 96-113 (RVELLKKEIDKFKDKKEV) of human kB-Ras1.

Immunogen Type

Synthetic peptide

Immunogen

NKIRAS1 antibody was raised against a synthetic peptide containing amino acid residues 96-113 (RVELLKKEIDKFKDKKEV) of human kB-Ras1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%); Bovine, Pig, Opossum, Turkey, Chicken (94%); Lizard, Xenopus (89%).

Antigen Modification

aa96-113

Product Categories/Family for anti-NKIRAS1 antibody

Family: Ras GTPase superfamily IPR001806

Applications Tested/Suitable for anti-NKIRAS1 antibody

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

Application Notes for anti-NKIRAS1 antibody

IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Other Notes

Small volumes of anti-NKIRAS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NKIRAS1. It may not necessarily be applicable to this product.

NCBI GI #

9966809

NCBI GeneID

28512

NCBI Accession #

NP_065078.1

UniProt Primary Accession #

Q9NYS0

UniProt Secondary Accession #

Q96K18

UniProt Related Accession #

Q9NYS0

GenBank Nucleotide #

NM_020345.3

NCBI Official Full Name

NF-kappa-B inhibitor-interacting Ras-like protein 1

NCBI Official Synonym Full Names

NFKB inhibitor interacting Ras-like 1

NCBI Official Symbol

NKIRAS1

NCBI Official Synonym Symbols

KBRAS1; kappaB-Ras1

UniProt Protein Name

NF-kappa-B inhibitor-interacting Ras-like protein 1

UniProt Synonym Protein Names

I-kappa-B-interacting Ras-like protein 1; Kappa B-Ras protein 1; KappaB-Ras1

Protein Family

NF-kappa-B inhibitor-interacting Ras-like protein

UniProt Gene Name

NKIRAS1

UniProt Synonym Gene Names

KBRAS1; Kappa B-Ras protein 1; KappaB-Ras1

UniProt Entry Name

KBRS1_HUMAN

NCBI Protein Information

NF-kappa-B inhibitor-interacting Ras-like protein 1; kappa B-ras 1; kappa B-Ras protein 1; I-kappa-B-interacting Ras-like protein 1; NFKB inhibitor interacting Ras-like protein 1

UniProt Comments for NKIRAS1

NKIRAS1: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

Protein type: G protein; G protein, monomeric, Ras; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction

Research Articles on NKIRAS1

1. Significant correlation was found between alterations of methylation status of miR-34b/c, miR-193a and the expression level of NKIRAS1 target gene in the primary lung tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NKIRAS1 antibody

Products by Pathway	Pathway Diagram
TNF-alpha/NF-kB Signaling Pathway antibodies	TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-NKIRAS1 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>2 publications with NKIRAS1 and Lung Neoplasms
Adenocarcinoma Antibodies	>2 publications with NKIRAS1 and Adenocarcinoma
Kidney Diseases Antibodies	>1 publications with NKIRAS1 and Kidney Diseases

Organs/Tissues associated with anti-NKIRAS1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>2 publications with NKIRAS1 and Lung
Placenta Antibodies	>1 publications with NKIRAS1 and Placenta
Kidney Antibodies	>1 publications with NKIRAS1 and Kidney