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UBE2L6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245393

MBS245393 is an antibody targeting UBE2L6. Also known as Mouse Monoclonal [clone K1H3] (IgG2b,k) to Human UBE2L6. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
UBE2L6, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone K1H3] (IgG2b,k) to Human UBE2L6
Product Gene Name
anti-UBE2L6 antibody
Product Synonym Names
Anti-UBE2L6 Antibody (aa1-152, clone K1H3) IHC-plus; UBE2L6; Ubiquitin carrier protein L6; Ubiquitin-protein ligase L6; UBCH8; RIG-B; Human UBE2L6
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
K1H3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
10,086 Da
OMIM
603890
3D Structure
ModBase 3D Structure for O14933
Target Species
Human
Immunogen Description
Recombinant human UBE2L6 (1-152aa) purified from E Coli. NCBI Accession No.: NP_004214.
Immunogen Type
Recombinant protein
Immunogen
UBE2L6 antibody was raised against recombinant human UBE2L6 (1-152aa) purified from E Coli. NCBI Accession No.: NP_004214.
Antigen Modification
aa1-152
Applications Tested/Suitable for anti-UBE2L6 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-UBE2L6 antibody
ELISA, IHC-P (5 ug/ml), WB (1:1000 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-UBE2L6 antibody

Anti-UBE2L6 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-UBE2L6 Monoclonal Antibody – Cat# MBS245393

Immunohistochemistry (IHC) of anti-UBE2L6 antibody

Anti-UBE2L6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-UBE2L6 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245393

Immunohistochemistry (IHC) of anti-UBE2L6 antibody

Anti-UBE2L6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-UBE2L6 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245393

Western Blot (WB) of anti-UBE2L6 antibody

Cell lysates of MCF7 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-UBE2L6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245393

Other Notes

Small volumes of anti-UBE2L6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2L6. It may not necessarily be applicable to this product.

NCBI GI #
4759282
NCBI GeneID
9246
NCBI Accession #
NP_004214.1
UniProt Primary Accession #
O14933
UniProt Secondary Accession #
Q8N5D8; Q9UEZ0; A6NDM6; A8MY53
UniProt Related Accession #
O14933
GenBank Nucleotide #
NM_004223.4
NCBI Official Full Name
ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2L 6
NCBI Official Symbol
UBE2L6
NCBI Official Synonym Symbols
RIG-B; UBCH8
UniProt Protein Name
Ubiquitin/ISG15-conjugating enzyme E2 L6
UniProt Synonym Protein Names
Retinoic acid-induced gene B protein; RIG-B; UbcH8; Ubiquitin carrier protein L6; Ubiquitin-protein ligase L6
Protein Family
Ubiquitin/ISG15-conjugating enzyme
UniProt Gene Name
UBE2L6
UniProt Synonym Gene Names
UBCH8; RIG-B
UniProt Entry Name
UB2L6_HUMAN
NCBI Protein Information
ubiquitin/ISG15-conjugating enzyme E2 L6; ubiquitin-protein ligase L6; ubiquitin carrier protein L6; retinoic acid induced gene B protein; retinoic acid-induced gene B protein

NCBI Summary for UBE2L6

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]
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UniProt Comments for UBE2L6

UBE2L6: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytosol

Molecular Function: protein binding; ISG15 conjugating enzyme activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein ubiquitination; innate immune response; protein modification process; negative regulation of interferon type I production
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Research Articles on UBE2L6

1. study reports that UBCH8 and UBE2G1 and UBE2G2 cooperate with CRL4Cdt2 in promoting the polyubiquitylation and subsequent degradation of p21 and Cdt1, respectively

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBE2L6 antibody

 Reference Product  PubMed Publications
 ISG15 antibody  >13 publications with UBE2L6 and ISG15
 STAT1 antibody  >1 publications with UBE2L6 and STAT1
 HERC5 antibody  >1 publications with UBE2L6 and HERC5
 DDX58 antibody  >1 publications with UBE2L6 and DDX58

Pathways associated with anti-UBE2L6 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram

Diseases associated with anti-UBE2L6 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >3 publications with UBE2L6 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with UBE2L6 and Inflammation
 Influenza, Human Antibodies  >2 publications with UBE2L6 and Influenza, Human
 Poisoning Antibodies  >1 publications with UBE2L6 and Poisoning
 Brain Diseases Antibodies  >1 publications with UBE2L6 and Brain Diseases
 Hypertrophy Antibodies  >1 publications with UBE2L6 and Hypertrophy
 Hyperplasia Antibodies  >1 publications with UBE2L6 and Hyperplasia
 Weight Loss Antibodies  >1 publications with UBE2L6 and Weight Loss

Organs/Tissues associated with anti-UBE2L6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with UBE2L6 and Blood
 Lung Antibodies  >2 publications with UBE2L6 and Lung
 Bone Antibodies  >1 publications with UBE2L6 and Bone
 Lymph Node Antibodies  >1 publications with UBE2L6 and Lymph Node
 Connective Tissue Antibodies  >1 publications with UBE2L6 and Connective Tissue
 Kidney Antibodies  >1 publications with UBE2L6 and Kidney
 Lymph Antibodies  >1 publications with UBE2L6 and Lymph
 Embryonic Tissue Antibodies  >1 publications with UBE2L6 and Embryonic Tissue
 Intestine Antibodies  >1 publications with UBE2L6 and Intestine
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