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ACOT11 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245451

MBS245451 is an antibody targeting ACOT11. Also known as Mouse Monoclonal [clone J4B2] (IgG2b,k) to Human ACOT11 / THEA. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
ACOT11 / THEA, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone J4B2] (IgG2b,k) to Human ACOT11 / THEA
Product Gene Name
Product Synonym Gene Name
THEA
Product Synonym Names
Anti-ACOT11 / THEA Antibody (aa19-250, clone J4B2) IHC-plus; ACOT11; BFIT2; Acyl-CoA thioesterase 11; KIAA0707; STARD14; THEM1; START domain containing 14; THEA; BFIT; BFIT1; Human ACOT11; THEA
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
J4B2
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide
Purity/Purification
Protein G Purified
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
67,152 Da
OMIM
606803
3D Structure
ModBase 3D Structure for Q8WXI4
Target Species
Human
Immunogen Description
Recombinant human ACOT11 (19-250aa) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
ACOT11 / THEA antibody was raised against recombinant human ACOT11 (19-250aa) purified from E Coli.
Antigen Modification
aa19-250
Applications Tested/Suitable for anti-ACOT11 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACOT11 antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ACOT11 antibody

Anti-ACOT11 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-ACOT11 Monoclonal Antibody – Cat# MBS245451

Immunohistochemistry (IHC) of anti-ACOT11 antibody

Anti-ACOT11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-ACOT11 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245451

Western Blot (WB) of anti-ACOT11 antibody

Cell lysates of LNCap, Hep3B (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ACOT11 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-ACOT11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245451

Other Notes

Small volumes of anti-ACOT11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-coenzyme A thioesterase 11 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 11
NCBI Official Symbol
ACOT11
NCBI Official Synonym Symbols
BFIT; THEA; THEM1; STARD14
UniProt Protein Name
Acyl-coenzyme A thioesterase 11
UniProt Synonym Protein Names
Acyl-CoA thioester hydrolase 11; Adipose-associated thioesterase; Brown fat-inducible thioesterase; BFIT
UniProt Gene Name
ACOT11
UniProt Synonym Gene Names
BFIT; KIAA0707; THEA; Acyl-CoA thioesterase 11; BFIT
UniProt Entry Name
ACO11_HUMAN
NCBI Protein Information
acyl-coenzyme A thioesterase 11; START domain containing 14; acyl-CoA thioester hydrolase 11; adipose-associated thioesterase; brown fat-inducible thioesterase; thioesterase, adipose associated; thioesterase superfamily member 1; StAR-related lipid transfer (START) domain containing 14

NCBI Summary for ACOT11

This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
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UniProt Comments for ACOT11

ACOT11: Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.-; Hydrolase

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: cytoplasm

Molecular Function: acyl-CoA hydrolase activity; lipid binding

Biological Process: response to temperature stimulus; response to cold; fatty acid metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-ACOT11 antibody

 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >4 publications with ACOT11 and Adipose Tissue
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