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GPNMB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245605

MBS245605 is an antibody targeting GPNMB. Also known as Anti-GPNMB/Osteoactivin Antibody; GPNMB / Osteoactivin. Reactive in Human. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPNMB, Monoclonal Antibody
Full Product Name
Anti-GPNMB/Osteoactivin Antibody; GPNMB / Osteoactivin
Product Gene Name
anti-GPNMB antibody
Product Synonym Names
Anti-GPNMB Antibody (clone 3A5) IHC-plus; GPNMB; Glycoprotein nmb-like protein; HGFIN; Glycoprotein NMB; Osteoactivin; Transmembrane glycoprotein NMB; Human GPNMB
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3A5
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4
Purity/Purification
Purified from ascites by Protein A
Concentration
0.5 mg/ml (lot specific)
Specificity
Human GPNMB
Preparation and Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
OMIM
604368
3D Structure
ModBase 3D Structure for Q14956
Immunogen Species
Antibody was raised against Human
Immunogen
GPNMB/Osteoactivin Antibody was raised against gPNMB (AAH32783.1, 1 a.a.~572 a.a) full length recombinant protein with GST tag
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-GPNMB antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GPNMB antibody
IHC-P (5 ug/ml), Western Blot: Recombinant protein.
Optimal dilution to be determined by the researcher

Immunohistochemistry (IHC) of anti-GPNMB antibody

Anti-GPNMB antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-GPNMB Monoclonal Antibody – Cat# MBS245605

Other Notes

Small volumes of anti-GPNMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPNMB. It may not necessarily be applicable to this product.

NCBI GI #
4505405
NCBI GeneID
10457
NCBI Accession #
NP_002501.1
UniProt Primary Accession #
Q14956
UniProt Secondary Accession #
Q6UVX1; Q8N1A1; A4D155
UniProt Related Accession #
Q14956
GenBank Nucleotide #
NM_002510.2
NCBI Official Full Name
transmembrane glycoprotein NMB isoform b
NCBI Official Synonym Full Names
glycoprotein (transmembrane) nmb
NCBI Official Symbol
GPNMB
NCBI Official Synonym Symbols
NMB; HGFIN
UniProt Protein Name
Transmembrane glycoprotein NMB
UniProt Synonym Protein Names
Transmembrane glycoprotein HGFIN
Protein Family
Transmembrane glycoprotein
UniProt Gene Name
GPNMB
UniProt Synonym Gene Names
HGFIN; NMB
UniProt Entry Name
GPNMB_HUMAN
NCBI Protein Information
transmembrane glycoprotein NMB; osteoactivin; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN

NCBI Summary for GPNMB

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPNMB

GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: integral to plasma membrane; integral to membrane; melanosome

Molecular Function: integrin binding; heparin binding

Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization
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Research Articles on GPNMB

1. Our data identify Gpnmb as a novel marker for obesity-induced adipose tissue macrophage infiltration and potentiator of interleukin-4 responses and point toward a crucial role for MITF in driving part of the adipose tissue macrophage phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPNMB antibody

 Reference Product  PubMed Publications
 TYRP1 antibody  >14 publications with GPNMB and TYRP1
 TREM2 antibody  >1 publications with GPNMB and TREM2
 FLCN antibody  >1 publications with GPNMB and FLCN
 TFE3 antibody  >1 publications with GPNMB and TFE3

Diseases associated with anti-GPNMB antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >18 publications with GPNMB and Disease Models, Animal
 Inflammation Antibodies  >17 publications with GPNMB and Inflammation
 Breast Neoplasms Antibodies  >11 publications with GPNMB and Breast Neoplasms
 Kidney Diseases Antibodies  >10 publications with GPNMB and Kidney Diseases
 Liver Diseases Antibodies  >10 publications with GPNMB and Liver Diseases
 Fibrosis Antibodies  >6 publications with GPNMB and Fibrosis
 Cardiovascular Diseases Antibodies  >5 publications with GPNMB and Cardiovascular Diseases
 Hypertension Antibodies  >5 publications with GPNMB and Hypertension
 Neoplasms, Experimental Antibodies  >5 publications with GPNMB and Neoplasms, Experimental
 Necrosis Antibodies  >4 publications with GPNMB and Necrosis

Organs/Tissues associated with anti-GPNMB antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >50 publications with GPNMB and Eye
 Blood Antibodies  >39 publications with GPNMB and Blood
 Bone Antibodies  >32 publications with GPNMB and Bone
 Kidney Antibodies  >15 publications with GPNMB and Kidney
 Nerve Antibodies  >14 publications with GPNMB and Nerve
 Brain Antibodies  >14 publications with GPNMB and Brain
 Liver Antibodies  >13 publications with GPNMB and Liver
 Skin Antibodies  >10 publications with GPNMB and Skin
 Muscle Antibodies  >10 publications with GPNMB and Muscle
 Lung Antibodies  >8 publications with GPNMB and Lung
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