Barbiturates ELISA Kit

Intended Uses: The BAFF, soluble (human) ELISA kit (hypersensitive) is to be used for the measurement of human BAFF in serum, plasma and cell culture supernatant. This ELISA Kit is for research use only.

Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human BAFF (BAFF) in serum, plasma and cell culture supernatant. A monoclonal antibody specific for BAFF has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, BAFF is recognized by the addition of a biotinylated monoclonal antibody specific for BAFF (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of BAFF in the samples!!Background/Introduction: BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a key survival factor for peripheral B cells. BAFF is a homotrimeric type II transmembrane protein that can be proteolytically processed by furin to be released as soluble cytokine (1). Soluble BAFF adopts the classical trimeric form of the TNF-family ligand. However, BAFF has the unique property among the TNF-ligand to assemble as a 60-mer (2). BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation (1). Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of persons with various autoimmune diseases (3), such as Sjögren's syndrome (4), Rheumatoid Arthritis (RA) (5), Multiple sclerosis (MS) (6) and Systemic Lupus Erythematosus (SLE) (7). BAFF is also increased levels in some lymphoid cancers (8).

Small volumes of Barbiturates elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580058 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Barbiturates, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Barbiturates. The ELISA analytical biochemical technique of the MBS580058 kit is based on Barbiturates antibody-Barbiturates antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Barbiturates antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Barbiturates. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).