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NEK2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245626

MBS245626 is an antibody targeting NEK2. Also known as Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human NEK2. Reactive in Human. Host: NEK2 antibody was produced in Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human NEK2
Product Gene Name
anti-NEK2 antibody
Product Synonym Names
Anti-NEK2 Antibody (clone 2F6) IHC-plus; NEK2; NIMA-related kinase 2; NEK2A; HsPK21; NimA-like protein kinase 1; NLK1; HSPK 21; NimA-related protein kinase 2; Human NEK2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F6
Host
NEK2 antibody was produced in Mouse
Species Reactivity
Human
Form/Format
PBS, pH7.4
Purity/Purification
Purified from ascites by protein A
Specificity
Human NEK2
Preparation and Storage
Short Term: 4°C
Long Term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
OMIM
gene 615565
3D Structure
ModBase 3D Structure for P51955
Target
NEK2
Family
Protein Kinase/ NEK
Immunogen Species
NEK 2 antibody was raised against Human
Antigen Type
Recombinant protein
Immunogen
NEK2 anitbody was raised against nEK2 (AAH43502, 331aa~445 aa) Partial recombinant protein with GST tag alone is 26KDa.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-NEK2 antibody

Family: Protein Kinase; Subfamily: NEK
Applications Tested/Suitable for anti-NEK2 antibody
Immunochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), Immunoprecipitaion (IP), ELISA (EIA)
Application Notes for anti-NEK2 antibody
IHC - Paraffin (5 ug/ml)

Immunohistochemistry (IHC) of anti-NEK2 antibody

Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-NEK2 Monoclonal Antibody – Cat# MBS245626

Western Blot (WB) of anti-NEK2 antibody

Western blot of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.
Mouse Anti-NEK2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245626

ELISA (EIA) of anti-NEK2 antibody

Detection limit for recombinant GST tagged NEK2 is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-NEK2 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245626

Other Notes

Small volumes of anti-NEK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK2. It may not necessarily be applicable to this product.

NCBI GI #
4505373
NCBI GeneID
4751
NCBI Accession #
NP_002488.1
UniProt Primary Accession #
P51955
UniProt Secondary Accession #
Q53FD6; Q5I1Z9; Q5VXZ1; Q6NZX8; Q7Z634; Q86XH2; Q96QN9
UniProt Related Accession #
P51955
GenBank Nucleotide #
NM_002497.3
NCBI Official Full Name
serine/threonine-protein kinase Nek2 isoform 1
NCBI Official Synonym Full Names
NIMA-related kinase 2
NCBI Official Symbol
NEK2
NCBI Official Synonym Symbols
NLK1; RP67; NEK2A; HsPK21; PPP1R111
UniProt Protein Name
Serine/threonine-protein kinase Nek2
UniProt Synonym Protein Names
HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1
Protein Family
Probable serine/threonine-protein kinase
UniProt Gene Name
NEK2
UniProt Synonym Gene Names
NEK2A; NLK1; NimA-related protein kinase 2
UniProt Entry Name
NEK2_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111; NIMA (never in mitosis gene a)-related kinase 2

NCBI Summary for NEK2

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
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UniProt Comments for NEK2

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Nucleolus; Kinase, protein; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: kinetochore; spindle pole; centrosome; microtubule; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; nucleolus; midbody; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; organelle organization and biogenesis; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle

Disease: Retinitis Pigmentosa 67
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Research Articles on NEK2

1. Data suggest a role for Nek2 driving centrosome amplification downstream of the E2F3 activator.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEK2 antibody

 Reference Product  PubMed Publications
 PLK1 antibody  >18 publications with NEK2 and PLK1
 CDK1 antibody  >9 publications with NEK2 and CDK1
 CEP250 antibody  >8 publications with NEK2 and CEP250
 NDC80 antibody  >7 publications with NEK2 and NDC80
 CDC20 antibody  >6 publications with NEK2 and CDC20
 BUB1B antibody  >3 publications with NEK2 and BUB1B

Pathways associated with anti-NEK2 antibody

 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram

Diseases associated with anti-NEK2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >25 publications with NEK2 and Breast Neoplasms
 Carcinoma Antibodies  >22 publications with NEK2 and Carcinoma
 Adenocarcinoma Antibodies  >15 publications with NEK2 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >10 publications with NEK2 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >7 publications with NEK2 and Lung Neoplasms
 Liver Neoplasms Antibodies  >6 publications with NEK2 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >4 publications with NEK2 and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >4 publications with NEK2 and Ovarian Neoplasms
 Carcinoma, Hepatocellular Antibodies  >4 publications with NEK2 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >4 publications with NEK2 and Liver Diseases

Organs/Tissues associated with anti-NEK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >10 publications with NEK2 and Lung
 Liver Antibodies  >9 publications with NEK2 and Liver
 Testis Antibodies  >8 publications with NEK2 and Testis
 Lymph Node Antibodies  >7 publications with NEK2 and Lymph Node
 Blood Antibodies  >6 publications with NEK2 and Blood
 Kidney Antibodies  >6 publications with NEK2 and Kidney
 Ovary Antibodies  >6 publications with NEK2 and Ovary
 Prostate Antibodies  >4 publications with NEK2 and Prostate
 Bone Antibodies  >4 publications with NEK2 and Bone
 Brain Antibodies  >3 publications with NEK2 and Brain
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