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Requiem / DPF2 (DPF2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245637

MBS245637 is an antibody targeting DPF2. Also known as Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human Requiem / DPF2. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Requiem / DPF2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human Requiem / DPF2
Product Gene Name
Product Synonym Names
Anti-Requiem / DPF2 Antibody (clone 2F6) IHC-plus; DPF2; BAF45D; REQ; UBID4; Zinc finger protein ubi-d4; BRG1-associated factor 45D; Ubi-d4; Protein requiem; Requiem; Human Requiem; DPF2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F6
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.43 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
23,599 Da
OMIM
601671
3D Structure
ModBase 3D Structure for Q92785
Target Species
Human
Immunogen Description
DPF2 (AAH14889, 56 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
Requiem / DPF2 antibody was raised against dPF2 (AAH14889, 56 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-DPF2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-DPF2 antibody
ELISA, IF (10 ug/ml), IHC-P (2.5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control LNCaP. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells.

Immunohistochemistry (IHC) of anti-DPF2 antibody

Anti-DPF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Mouse Anti-DPF2 Monoclonal Antibody – Cat# MBS245637

Immunohistochemistry (IHC) of anti-DPF2 antibody

Anti-DPF2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Mouse Anti-DPF2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245637

Immunofluorescence (IF) of anti-DPF2 antibody

Immunofluorescence of monoclonal antibody to DPF2 on HeLa cell (antibody concentration 10 ug/ml).
Mouse Anti-DPF2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245637

Western Blot (WB) of anti-DPF2 antibody

Western blot of DPF2 expression in transfected 293T cell line by DPF2 monoclonal antibody clone 2F6.
Mouse Anti-DPF2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245637

Western Blot (WB) of anti-DPF2 antibody

DPF2 monoclonal antibody clone 2F6 Western blot of DPF2 expression in LNCaP.
Mouse Anti-DPF2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245637

Other Notes

Small volumes of anti-DPF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DPF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein ubi-d4
NCBI Official Synonym Full Names
D4, zinc and double PHD fingers family 2
NCBI Official Symbol
DPF2
NCBI Official Synonym Symbols
REQ; UBID4; ubi-d4
UniProt Protein Name
Zinc finger protein ubi-d4
UniProt Synonym Protein Names
Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4, zinc and double PHD fingers family 2; Protein requiem
Protein Family
UniProt Gene Name
DPF2
UniProt Synonym Gene Names
BAF45D; REQ; UBID4; BAF45D
UniProt Entry Name
REQU_HUMAN
NCBI Protein Information
zinc finger protein ubi-d4; BAF45D; protein requiem; BRG1-associated factor 45D; apoptosis response zinc finger protein; requiem, apoptosis response zinc finger

NCBI Summary for DPF2

The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]
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UniProt Comments for DPF2

requiem: May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells. Belongs to the requiem/DPF family.

Protein type: Apoptosis; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm

Molecular Function: zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DPF2 antibody

Diseases associated with anti-DPF2 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with DPF2 and Necrosis
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