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TIGIT Recombinant Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488486

Recombinant monoclonal antibody to TIGIT. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 1B4. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIGIT, Recombinant Antibody
Full Product Name
Anti-TIGIT [1B4]
Product Gene Name
TIGIT recombinant antibody
Product Synonym Names
VSTM3; T cell Ig and ITIM domain; T cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain-containing protein 3; WUCAM
Clonality
Monoclonal
Isotype
Rabbit IgG lambda
Clone Number
1B4
Species Reactivity
Mouse
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Purified
Specificity
This antibody is specific for murine TIGIT.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
3D Structure
ModBase 3D Structure for P86176
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Antibody Species
Rabbit
Original Species Name
Mouse
Immunogen
This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).
Buffer Composition
PBS only with 0.02% Proclin 300.

Product Categories/Family for TIGIT recombinant antibody

Autoimmunity; Oncology; Synergy
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for TIGIT recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Blocking (BL)

Western Blot (WB) of TIGIT recombinant antibody

Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Anti-TIGIT Monoclonal Recombinant Antibody – Cat# MBS488486

Product References and Citations for TIGIT recombinant antibody

Dixon et al. Functional Anti-TIGIT Antibodies Regulate Development of Autoimmunity and Antitumor Immunity J Immunol. 2018 Apr 15;200(8):3000-3007 PMID:29500245
Original Published Journal: J Immunol. 2018 Apr 15;200(8):3000-3007
Publication Note: Describes the original generation of this antibody, and the establishment of its TIGIT-blocking function.

Other Notes

Small volumes of TIGIT recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIGIT purchase

MBS488486 is a recombinant antibody targeting TIGIT. Also known as Anti-TIGIT [1B4]. Reactive in Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TIGIT. It may not necessarily be applicable to this product.

NCBI GI #
224493410
NCBI GeneID
100043314
NCBI Accession #
P86176.1
UniProt Primary Accession #
P86176
UniProt Related Accession #
P86176
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
Tigit
NCBI Official Synonym Symbols
Vstm3
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
UniProt Synonym Protein Names
V-set and transmembrane domain-containing protein 3
Protein Family
T-cell immunoreceptor with Ig and ITIM domains
UniProt Gene Name
Tigit
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains

UniProt Comments for TIGIT

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Research Articles on TIGIT

1. Results indicate that TIGIT enhanced on tolerant T cells may function as a ligand of its paired receptor CD155 to transduce signaling into its expressing naive T cells to accelerate new TIGIT expressions as well as IL-2 suppression via B lymphocyte-induced maturation protein 1 enhancement.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TIGIT recombinant antibody

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