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PRPK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246033

MBS246033 is an antibody targeting PRPK. Also known as Mouse Monoclonal [clone 4B9-1H3] (IgG1,k) to Human PRPK / TP53RK. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PRPK / TP53RK, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4B9-1H3] (IgG1,k) to Human PRPK / TP53RK
Product Gene Name
Product Synonym Gene Name
TP53RK
Product Synonym Names
Anti-PRPK / TP53RK Antibody (clone 4B9-1H3) IHC-plus; TP53RK; C20orf64; BUD32; DJ101A2.2; DJ101A2; Nori-2p; p53-related protein kinase; PRPK; TP53-regulating kinase; Nori-2; TP53 regulating kinase; Human PRPK; TP53RK
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B9-1H3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
28,160 Da
OMIM
608679
3D Structure
ModBase 3D Structure for Q96S44
Target Species
Human
Immunogen Description
TP53RK (AAH09727, 1 a.a. ~ 254 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
PRPK / TP53RK antibody was raised against tP53RK (AAH09727, 1 a.a. ~ 254 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-PRPK antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PRPK antibody
ELISA, IHC-P (10 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-PRPK antibody

Anti-TP53RK antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-PRPK Monoclonal Antibody – Cat# MBS246033

Western Blot (WB) of anti-PRPK antibody

TP53RK monoclonal antibody, clone 4B9-1H3 Western blot of TP53RK expression in Jurkat.
Mouse Anti-PRPK Monoclonal Antibody, Western Blot (WB) – Cat# MBS246033

ELISA (EIA) of anti-PRPK antibody

Detection limit for recombinant GST tagged TP53RK is approximately 1 ng/ml as a capture antibody.
Mouse Anti-PRPK Monoclonal Antibody, ELISA (EIA) – Cat# MBS246033

Other Notes

Small volumes of anti-PRPK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRPK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
TP53-regulating kinase
NCBI Official Synonym Full Names
TP53 regulating kinase
NCBI Official Symbol
TP53RK
NCBI Official Synonym Symbols
PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
UniProt Protein Name
TP53-regulating kinase
UniProt Synonym Protein Names
Atypical serine/threonine protein kinase TP53RK; EKC/KEOPS complex subunit TP53RK (EC:3.6.-.-); Nori-2; p53-related protein kinase
UniProt Gene Name
TP53RK
UniProt Synonym Gene Names
C20orf64; PRPK
UniProt Entry Name
PRPK_HUMAN
NCBI Protein Information
TP53-regulating kinase; p53-related protein kinase; EKC/KEOPS complex subunit TP53RK; atypical serine/threonine protein kinase TP53RK

UniProt Comments for PRPK

PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Other group; Bud32 family

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; hydrolase activity; p53 binding; ATP binding

Biological Process: tRNA processing; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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