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ACOT9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246421

MBS246421 is an antibody targeting ACOT9. Also known as Mouse Monoclonal [clone 4E4] (IgG1,k) to Human ACOT9. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ACOT9, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4E4] (IgG1,k) to Human ACOT9
Product Gene Name
Product Synonym Names
Anti-ACOT9 Antibody (clone 4E4) IHC-plus; ACOT9; ACATE2; Acyl-CoA thioester hydrolase 9; Acyl-CoA thioesterase 9; MTACT48; CGI-16; MT-ACT48; Human ACOT9
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4E4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
50,851 Da
OMIM
300862
3D Structure
ModBase 3D Structure for Q9Y305
Target Species
Human
Immunogen Description
ACATE2 (AAH12573, 1 a.a. ~ 213 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
ACOT9 antibody was raised against aCATE2 (AAH12573, 1 a.a. ~ 213 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-ACOT9 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACOT9 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-ACOT9 antibody

Anti-ACOT9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-ACOT9 Monoclonal Antibody – Cat# MBS246421

Immunohistochemistry (IHC) of anti-ACOT9 antibody

Anti-ACOT9 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-ACOT9 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246421

Immunofluorescence (IF) of anti-ACOT9 antibody

Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell (antibody concentration 10 ug/ml).
Mouse Anti-ACOT9 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246421

Western Blot (WB) of anti-ACOT9 antibody

Western blot of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody, clone 4E4.
Mouse Anti-ACOT9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246421

Western Blot (WB) of anti-ACOT9 antibody

ACATE2 monoclonal antibody, clone 4E4 Western blot of ACATE2 expression in MCF-7.
Mouse Anti-ACOT9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246421

ELISA (EIA) of anti-ACOT9 antibody

Detection limit for recombinant GST tagged ACOT9 is approximately 3 ng/ml as a capture antibody.
Mouse Anti-ACOT9 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246421

Other Notes

Small volumes of anti-ACOT9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-coenzyme A thioesterase 9, mitochondrial isoform b
NCBI Official Synonym Full Names
acyl-CoA thioesterase 9
NCBI Official Symbol
ACOT9
NCBI Official Synonym Symbols
ACATE2; CGI-16; MTACT48; MT-ACT48
UniProt Protein Name
Acyl-coenzyme A thioesterase 9, mitochondrial
UniProt Synonym Protein Names
Acyl-CoA thioester hydrolase 9
UniProt Gene Name
ACOT9
UniProt Synonym Gene Names
Acyl-CoA thioesterase 9
UniProt Entry Name
ACOT9_HUMAN
NCBI Protein Information
acyl-coenzyme A thioesterase 9, mitochondrial; acyl-CoA thioester hydrolase 9; mitochondrial Acyl-CoA Thioesterase; acyl-Coenzyme A thioesterase 2, mitochondrial

NCBI Summary for ACOT9

The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

UniProt Comments for ACOT9

ACOT9: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs. Belongs to the acyl coenzyme A hydrolase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.2.-

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: mitochondrion

Molecular Function: acetyl-CoA hydrolase activity

Biological Process: acyl-CoA metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACOT9 antibody

Organs/Tissues associated with anti-ACOT9 antibody

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