Mouse Anti-TUBB3 Monoclonal Antibody – Cat# MBS246676

Anti-TUBB3 / Beta III Tubulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

TUBB3 Antibody – Mouse Monoclonal

Name: TUBB3 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS246676
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246676

MBS246676 is an antibody targeting TUBB3. Also known as Mouse Monoclonal (IgG2a) to Human TUBB3 / Tubulin Beta 3. Reactive in Gibbon, Bovine, Dog, Gorilla, Human, Monkey, PigPredicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity).… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TUBB3 / Tubulin Beta 3, Monoclonal Antibody

Full Product Name

Mouse Monoclonal (IgG2a) to Human TUBB3 / Tubulin Beta 3

Product Gene Name

anti-TUBB3 antibody

Product Synonym Names

Anti-TUBB3 / Tubulin Beta 3 Antibody (aa436-450) IHC-plus; TUBB3; CDCBM; Beta III Tubulin; Class III beta-tubulin; TUBB4; Tubulin; beta 3; Tubulin beta-III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin; beta 3 class III; CFEOM3A; Human TUBB3; Tubulin Beta 3

Clonality

Monoclonal

Isotype

IgG2a

Host

Mouse

Species Reactivity

Gibbon, Bovine, Dog, Gorilla, Human, Monkey, Pig
Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)

Form/Format

0.1 M Tris-glycine, 0.15 M sodium chloride, pH 7.4, 0.05% sodium azide, 30% glycerol. Sourced in TCS.

Purity/Purification

Protein G Purified

Concentration

0.99 mg/ml (lot specific)

Specificity

Recognizes human neuronal specific beta3 tubulin. Species cross-reactivity: Mouse, rat and bovine. Broad species cross-reactivity predicted based on conservation of immunogen sequence.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12months.

Molecular Weight

42,433 Da

OMIM

gene 614039

3D Structure

ModBase 3D Structure for Q13509

Target Species

Human

Immunogen Description

Synthetic peptide corresponding to amino acids 436-450 (C-MYEDDEEESEAQGPK) of neuronal specific beta3 tubulin (KLH coupled). Epitope: EAQGPK. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Pig (100%).

Immunogen Type

Synthetic peptide - KLH conjugated

Immunogen

TUBB3 / Tubulin Beta 3 antibody was raised against synthetic peptide corresponding to amino acids 436-450 (C-MYEDDEEESEAQGPK) of neuronal specific beta3 tubulin (KLH coupled). Epitope: EAQGPK. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Pig (100%); Mouse, Rat, Hamster, Platypus (93%); Xenopus (87%).

Antigen Modification

aa436-450

Product Categories/Family for anti-TUBB3 antibody

Family: Tubulin

Applications Tested/Suitable for anti-TUBB3 antibody

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-TUBB3 antibody

ICC, IHC-P (10 ug/ml), WB (0.05 - 1 ug/ml)
Usage: Suitable for use in Immunocytochemistry and Western Blot. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Other Notes

Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB3. It may not necessarily be applicable to this product.

NCBI GI #

50592996

NCBI GeneID

10381

NCBI Accession #

NP_006077.2

UniProt Primary Accession #

Q13509

UniProt Secondary Accession #

Q9BTZ0; Q9BW10; A8K854

UniProt Related Accession #

Q13509

GenBank Nucleotide #

NM_006086.3

NCBI Official Full Name

tubulin beta-3 chain isoform 1

NCBI Official Synonym Full Names

tubulin, beta 3 class III

NCBI Official Symbol

TUBB3

NCBI Official Synonym Symbols

CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A

UniProt Protein Name

Tubulin beta-3 chain

UniProt Synonym Protein Names

Tubulin beta-4 chain; Tubulin beta-III

Protein Family

Tubulin

UniProt Gene Name

TUBB3

UniProt Synonym Gene Names

TUBB4

UniProt Entry Name

TBB3_HUMAN

NCBI Protein Information

tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin

NCBI Summary for TUBB3

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

UniProt Comments for TUBB3

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: microtubule; cell soma; axon; dendrite; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding

Biological Process: protein polymerization; axon guidance; mitosis; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1

Research Articles on TUBB3

1. Data indicate that ABCB1 protein, beta tubulin I and III (betaI, and betaIII tubulin) might contribute to the multidrug resistance (MDR) of MCF7/DOC and be potential therapeutic targets for overcoming MDR of breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBB3 antibody

Reference Product	PubMed Publications
POMC antibody	>3 publications with TUBB3 and POMC

Pathways associated with anti-TUBB3 antibody

Products by Pathway	Pathway Diagram
Chaperonin-mediated Protein Folding Pathway antibodies	Chaperonin-mediated Protein Folding Pathway Diagram
Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies	Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies	Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies	Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Orphan Transporters Pathway antibodies	Orphan Transporters Pathway Diagram
Parkin-Ubiquitin Proteasomal System Pathway antibodies	Parkin-Ubiquitin Proteasomal System Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-TUBB3 antibody

Disease Name	Pubmed Publications
Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement Antibodies	>573 publications with TUBB3 and Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement
Nervous System Diseases Antibodies	>71 publications with TUBB3 and Nervous System Diseases
Brain Diseases Antibodies	>38 publications with TUBB3 and Brain Diseases
Breast Neoplasms Antibodies	>30 publications with TUBB3 and Breast Neoplasms
Ovarian Neoplasms Antibodies	>25 publications with TUBB3 and Ovarian Neoplasms
Eye Diseases Antibodies	>19 publications with TUBB3 and Eye Diseases
Disease Models, Animal Antibodies	>19 publications with TUBB3 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>10 publications with TUBB3 and Neoplasms, Experimental
Nerve Degeneration Antibodies	>9 publications with TUBB3 and Nerve Degeneration
Cardiovascular Diseases Antibodies	>7 publications with TUBB3 and Cardiovascular Diseases

Organs/Tissues associated with anti-TUBB3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>122 publications with TUBB3 and Brain
Nerve Antibodies	>88 publications with TUBB3 and Nerve
Lung Antibodies	>62 publications with TUBB3 and Lung
Eye Antibodies	>39 publications with TUBB3 and Eye
Embryonic Tissue Antibodies	>31 publications with TUBB3 and Embryonic Tissue
Blood Antibodies	>28 publications with TUBB3 and Blood
Muscle Antibodies	>27 publications with TUBB3 and Muscle
Bone Antibodies	>16 publications with TUBB3 and Bone
Vascular Antibodies	>16 publications with TUBB3 and Vascular
Ganglia Antibodies	>15 publications with TUBB3 and Ganglia