• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DEFA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246828

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract,… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
DEFA1 / HP1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human DEFA1 / HP1
Product Gene Name
Product Synonym Gene Name
HP1
Product Synonym Names
Anti-DEFA1 / HP1 Antibody (Internal) IHC-plus; DEFA1; DEFA2; Defensin; alpha 2; HP-1; HP1; Myeloid-related sequence; MRS; DEF1; Defensin; alpha 1; HNP-1; Neutrophil defensin 1; Human DEFA1; HP1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Chimpanzee, Gorilla, Human
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human DEFA1. This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
10,201 Da
OMIM
125220
3D Structure
ModBase 3D Structure for P59665
Target Species
Human
Immunogen Description
Synthetic peptide C-DESLAPKHPGSRKN from an internal region of human DEFA1 (NP_004075.1; NP_005208.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon (86%).
Immunogen Type
Synthetic peptide
Immunogen
DEFA1 / HP1 antibody was raised against synthetic peptide C-DESLAPKHPGSRKN from an internal region of human DEFA1 (NP_004075.1; NP_005208.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon (86%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-DEFA1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-DEFA1 antibody
ELISA (1:128000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown ident...

Immunohistochemistry (IHC) of anti-DEFA1 antibody

Anti-DEFA1 / NP-1 antibody IHC of human thymus, myeloid cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-DEFA1 Polyclonal Antibody – Cat# MBS246828

Western Blot (WB) of anti-DEFA1 antibody

DEFA1 antibody (2 ug/ml) staining of Human Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DEFA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS246828

Other Notes

Small volumes of anti-DEFA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFA1 purchase

MBS246828 is an antibody targeting DEFA1. Also known as Goat Polyclonal to Human DEFA1 / HP1. Reactive in Chimpanzee, Gorilla, Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for DEFA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neutrophil defensin 1 preproprotein
NCBI Official Synonym Full Names
defensin, alpha 1
NCBI Official Symbol
DEFA1
NCBI Official Synonym Symbols
HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
UniProt Protein Name
Neutrophil defensin 1
UniProt Synonym Protein Names
Defensin, alpha 1; HNP-1; HP-1; HP1
Protein Family
UniProt Gene Name
DEFA1
UniProt Synonym Gene Names
DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
UniProt Entry Name
DEF1_HUMAN
NCBI Protein Information
neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence

NCBI Summary for DEFA1

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]
Show more

UniProt Comments for DEFA1

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000