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ACO2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247001

Aconitase 2 is an enzyme of the TCA cycle and is encoded in the nucleus and active within the mitochondrion. Aconitase catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. Isoforms of ACO2 have been… More details

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Product Overview

Product Name
ACO2 / Aconitase 2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human ACO2 / Aconitase 2
Product Gene Name
Product Synonym Names
Anti-ACO2 / Aconitase 2 Antibody (Internal) IHC-plus; ACO2; ACONM; Aconitase 2; Aconitase 2; mitochondrial; Citrate hydro-lyase; ICRD; Human ACO2; Aconitase 2
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Chicken, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ACO2.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
85,425 Da
OMIM
gene 614559
3D Structure
ModBase 3D Structure for Q99798
Target Species
Human
Immunogen Description
Synthetic peptide C-QHVDVSPTSQRLQ from an internal region of human ACO2 (NP_001089.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Turkey, Chicken (100%).
Immunogen Type
Synthetic peptide
Immunogen
ACO2 / Aconitase 2 antibody was raised against synthetic peptide C-QHVDVSPTSQRLQ from an internal region of human ACO2 (NP_001089.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Turkey, Chicken (100%); Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Platypus (92%); Elephant, Xenopus, Beetle (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-ACO2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACO2 antibody
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.01 - 0.03 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 ug/ml.

Immunohistochemistry (IHC) of anti-ACO2 antibody

Anti-ACO2 / Aconitase 2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-ACO2 Polyclonal Antibody – Cat# MBS247001

Western Blot (WB) of anti-ACO2 antibody

ACO2 antibody (0.01 ug/ml) staining of Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ACO2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247001

Other Notes

Small volumes of anti-ACO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACO2 purchase

MBS247001 is an antibody targeting ACO2. Also known as Goat Polyclonal to Human ACO2 / Aconitase 2. Reactive in Gibbon, Chicken, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ACO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
aconitate hydratase, mitochondrial
NCBI Official Synonym Full Names
aconitase 2, mitochondrial
NCBI Official Symbol
ACO2
NCBI Official Synonym Symbols
ICRD; ACONM
UniProt Protein Name
Aconitate hydratase, mitochondrial
UniProt Synonym Protein Names
Citrate hydro-lyase
UniProt Gene Name
ACO2
UniProt Synonym Gene Names
Aconitase
UniProt Entry Name
ACON_HUMAN
NCBI Protein Information
aconitate hydratase, mitochondrial; citrate hydro-lyase

NCBI Summary for ACO2

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
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UniProt Comments for ACO2

ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Mitochondrial; EC 4.2.1.3; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function: iron ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; 3 iron, 4 sulfur cluster binding

Biological Process: isocitrate metabolic process; cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; citrate metabolic process

Disease: Infantile Cerebellar-retinal Degeneration; Optic Atrophy 8
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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