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RAMA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247109

This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome… More details

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Product Overview

Online Datasheet in PDF
Product Name
RAMA1 / SKA3, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human RAMA1 / SKA3
Product Gene Name
anti-RAMA1 antibody
Product Synonym Gene Name
SKA3
Product Synonym Names
Anti-RAMA1 / SKA3 Antibody (C-Terminus) IHC-plus; SKA3; C13orf3; RAMA1; Human RAMA1; SKA3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SKA3
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
43,978 Da
3D Structure
ModBase 3D Structure for Q8IX90
Target Species
Human
Immunogen Description
A 13 amino acid peptide from near the carboxy terminus of human SKA3.
Immunogen Type
Synthetic peptide
Immunogen
RAMA1 / SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-RAMA1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RAMA1 antibody
ELISA, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5-1 ug/ml.

Immunohistochemistry (IHC) of anti-RAMA1 antibody

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RAMA1 Polyclonal Antibody – Cat# MBS247109

Western Blot (WB) of anti-RAMA1 antibody

Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Rabbit Anti-RAMA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247109

Other Notes

Small volumes of anti-RAMA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAMA1 purchase

MBS247109 is an antibody targeting RAMA1. Also known as Rabbit Polyclonal to Human RAMA1 / SKA3. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAMA1. It may not necessarily be applicable to this product.

NCBI GI #
260763910
NCBI GeneID
221150
NCBI Accession #
NP_659498.4
UniProt Primary Accession #
Q8IX90
UniProt Secondary Accession #
Q5VZV5; Q86WR2; Q8NBG1; Q96D22; A2A330; A2A331; B2RBY2
UniProt Related Accession #
Q8IX90
GenBank Nucleotide #
NM_145061.5
NCBI Official Full Name
spindle and kinetochore-associated protein 3 isoform 1
NCBI Official Synonym Full Names
spindle and kinetochore associated complex subunit 3
NCBI Official Symbol
SKA3
NCBI Official Synonym Symbols
RAMA1; C13orf3
UniProt Protein Name
Spindle and kinetochore-associated protein 3
UniProt Gene Name
SKA3
UniProt Synonym Gene Names
C13orf3; RAMA1
UniProt Entry Name
SKA3_HUMAN
NCBI Protein Information
spindle and kinetochore-associated protein 3

UniProt Comments for RAMA1

RAMA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Belongs to the SKA3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: spindle microtubule; cytoplasm; outer kinetochore of condensed chromosome

Molecular Function: protein binding

Biological Process: mitosis; cell division; regulation of microtubule polymerization or depolymerization; chromosome segregation
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Research Articles on RAMA1

1. The structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAMA1 antibody

 Reference Product  PubMed Publications
 SKA1 antibody  >10 publications with RAMA1 and SKA1
 SKA2 antibody  >9 publications with RAMA1 and SKA2
 NDC80 antibody  >4 publications with RAMA1 and NDC80
 CDK1 antibody  >1 publications with RAMA1 and CDK1

Diseases associated with anti-RAMA1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >1 publications with RAMA1 and Breast Neoplasms
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