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AFF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248079

MBS248079 is an antibody targeting AFF1. Also known as Rabbit Polyclonal to Human AFF1 / AF4. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
AFF1 / AF4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human AFF1 / AF4
Product Gene Name
Product Synonym Gene Name
AF4
Product Synonym Names
Anti-AFF1 / AF4 Antibody (aa1180-1210) IHC-plus; FMR2 family; member 1; AF-4; AF4; AF4/FMR2 family member 1; FEL; PBM1; Protein FEL; MLLT2; Unknown; Protein AF-4; Proto-oncogene AF4; Human AFF1; AF4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human AFF1 / AF4
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
131,578 Da
OMIM
159557
3D Structure
ModBase 3D Structure for P51825
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human MLLT2.
Immunogen Type
Synthetic peptide
Immunogen
AFF1 / AF4 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human MLLT2.
Antigen Modification
aa1180-1210
Applications Tested/Suitable for anti-AFF1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-AFF1 antibody
IHC-P (5 ug/ml), WB (1:1000)

Immunohistochemistry (IHC) of anti-AFF1 antibody

Anti-AFF1 / AF4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-AFF1 Polyclonal Antibody – Cat# MBS248079

Western Blot (WB) of anti-AFF1 antibody

MLLT2 Antibody Western blot of K562 cell line lysates (35 ug/lane). This demonstrates the MLLT2 antibody detected the MLLT2 protein (arrow).
Rabbit Anti-AFF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248079

Other Notes

Small volumes of anti-AFF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AFF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
AF4/FMR2 family member 1 isoform 2
NCBI Official Synonym Full Names
AF4/FMR2 family, member 1
NCBI Official Symbol
AFF1
NCBI Official Synonym Symbols
AF4; PBM1; MLLT2
UniProt Protein Name
AF4/FMR2 family member 1
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4
Protein Family
UniProt Gene Name
AFF1
UniProt Synonym Gene Names
AF4; FEL; MLLT2; PBM1; Protein AF-4
UniProt Entry Name
AFF1_HUMAN
NCBI Protein Information
AF4/FMR2 family member 1; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2

UniProt Comments for AFF1

AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: transcription elongation factor complex

Molecular Function: protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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