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Ceramide Kinase / CERK (CERK) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248447

MBS248447 is an antibody targeting CERK. Also known as Rabbit Polyclonal to Human Ceramide Kinase / CERK. Reactive in Human, Mouse. Host: Rabbit. More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ceramide Kinase / CERK, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human Ceramide Kinase / CERK
Product Gene Name
anti-CERK antibody
Product Synonym Names
CERK , Acylsphingosine kinase, Ceramide kinase, DA59H18.2, DA59H18.3, KIAA1646, Lipid kinase 4, Lipid kinase LK4, HCERK, LK4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Specificity
This CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human CERK.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
OMIM
610307
3D Structure
ModBase 3D Structure for Q8TCT0
Family/Subfamily
Non-Protein Kinase/ Sphingosine
Immunogen
Ceramide Kinase / CERK antibody was raised against human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CERK antibody

Family: Non-Protein Kinase; Subfamily: Sphingosine
Applications Tested/Suitable for anti-CERK antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-CERK antibody
IHC-P (1:50), WB (1:1000)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-CERK antibody

Anti-Ceramide Kinase / CERK antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Rabbit Anti-CERK Polyclonal Antibody – Cat# MBS248447

Western Blot (WB) of anti-CERK antibody

The anti-CERK antibody is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).
Rabbit Anti-CERK Polyclonal Antibody, Western Blot (WB) – Cat# MBS248447

Other Notes

Small volumes of anti-CERK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CERK. It may not necessarily be applicable to this product.

NCBI GI #
20336726
NCBI GeneID
64781
NCBI Accession #
NP_073603.2
UniProt Primary Accession #
Q8TCT0
UniProt Secondary Accession #
Q6NX59; Q9BYB3; Q9UGE5; A0JNT4; A8K611
UniProt Related Accession #
Q8TCT0
GenBank Nucleotide #
NM_022766.5
NCBI Official Full Name
ceramide kinase
NCBI Official Synonym Full Names
ceramide kinase
NCBI Official Symbol
CERK
NCBI Official Synonym Symbols
LK4; hCERK; dA59H18.2; dA59H18.3
UniProt Protein Name
Ceramide kinase
UniProt Synonym Protein Names
Acylsphingosine kinase; Lipid kinase 4; LK4
Protein Family
Ceramide kinase
UniProt Gene Name
CERK
UniProt Synonym Gene Names
KIAA1646; hCERK; LK4
UniProt Entry Name
CERK1_HUMAN
NCBI Protein Information
ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase

NCBI Summary for CERK

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

UniProt Comments for CERK

CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.

Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.138

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrion; integral to membrane; plasma membrane

Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport
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Research Articles on CERK

1. Data suggest that inhibition of ceramide kinase (CerK) [by specific gene silencing or pharmacological inhibition] drastically reduced cell proliferation in a neuroblastoma cell line.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CERK antibody

 Reference Product  PubMed Publications
 SMPD1 antibody  >1 publications with CERK and SMPD1

Pathways associated with anti-CERK antibody

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-CERK antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >15 publications with CERK and Inflammation
 Necrosis Antibodies  >4 publications with CERK and Necrosis
 Disease Models, Animal Antibodies  >4 publications with CERK and Disease Models, Animal
 Breast Neoplasms Antibodies  >2 publications with CERK and Breast Neoplasms
 Insulin Resistance Antibodies  >2 publications with CERK and Insulin Resistance
 Kidney Diseases Antibodies  >2 publications with CERK and Kidney Diseases
 Liver Diseases Antibodies  >1 publications with CERK and Liver Diseases
 Movement Disorders Antibodies  >1 publications with CERK and Movement Disorders
 Glucose Intolerance Antibodies  >1 publications with CERK and Glucose Intolerance
 Adenocarcinoma Antibodies  >1 publications with CERK and Adenocarcinoma

Organs/Tissues associated with anti-CERK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with CERK and Blood
 Bone Antibodies  >5 publications with CERK and Bone
 Brain Antibodies  >5 publications with CERK and Brain
 Eye Antibodies  >4 publications with CERK and Eye
 Vascular Antibodies  >4 publications with CERK and Vascular
 Bone Marrow Antibodies  >4 publications with CERK and Bone Marrow
 Liver Antibodies  >3 publications with CERK and Liver
 Kidney Antibodies  >3 publications with CERK and Kidney
 Spleen Antibodies  >3 publications with CERK and Spleen
 Skin Antibodies  >2 publications with CERK and Skin
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