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ATG14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248588

MBS248588 is an antibody targeting ATG14. Also known as Rabbit Polyclonal to Human ATG14. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ATG14, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human ATG14
Product Gene Name
anti-ATG14 antibody
Product Synonym Names
Anti-ATG14 Antibody (Internal) IHC-plus; ATG14; Autophagy related 14; Beclin 1-Interacting protein; KIAA0831; ATG14L; BARKOR; Human ATG14
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human ATG14. At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Molecular Weight
42,434 Da
OMIM
613515
3D Structure
ModBase 3D Structure for Q6ZNE5
Target Species
Human
Immunogen Description
An 18 amino acid peptide near the center of human ATG14.
Immunogen Type
Synthetic peptide
Immunogen
ATG14 antibody was raised against an 18 amino acid peptide near the center of human ATG14.
Antigen Modification
Internal
Applications Tested/Suitable for anti-ATG14 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-ATG14 antibody
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: ATG14 antibody can be used for detection of ATG14 by Western blot at 1-2 ug/mL.

Immunohistochemistry (IHC) of anti-ATG14 antibody

Anti-ATG14 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-ATG14 Polyclonal Antibody – Cat# MBS248588

Immunofluorescence (IF) of anti-ATG14 antibody

Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/ml.
Rabbit Anti-ATG14 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS248588

Western Blot (WB) of anti-ATG14 antibody

Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-ATG14 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248588

Other Notes

Small volumes of anti-ATG14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG14. It may not necessarily be applicable to this product.

NCBI GI #
50540545
NCBI GeneID
22863
NCBI Accession #
NP_055739.2
UniProt Primary Accession #
Q6ZNE5
UniProt Secondary Accession #
O94920; Q32MK7; Q32MK8; A6NJE4; A8K9U5; B7ZWP5
UniProt Related Accession #
Q6ZNE5
GenBank Nucleotide #
NM_014924.4
NCBI Official Full Name
beclin 1-associated autophagy-related key regulator
NCBI Official Synonym Full Names
autophagy related 14
NCBI Official Symbol
ATG14
NCBI Official Synonym Symbols
ATG14L; BARKOR; KIAA0831
UniProt Protein Name
Beclin 1-associated autophagy-related key regulator
UniProt Synonym Protein Names
Autophagy-related protein 14-like protein; Atg14L
Protein Family
Autophagy-related protein
UniProt Gene Name
ATG14
UniProt Synonym Gene Names
KIAA0831; Barkor; Atg14L
UniProt Entry Name
BAKOR_HUMAN
NCBI Protein Information
beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; ATG14 autophagy related 14 homolog; autophagy-related protein 14-like protein

UniProt Comments for ATG14

Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: nucleoplasm; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; phagocytic vesicle; axoneme; nucleus

Molecular Function: protein binding

Biological Process: regulation of protein amino acid phosphorylation; macroautophagy; mitochondrion degradation; endosome to lysosome transport; positive regulation of autophagy; autophagic vacuole formation
Show more

Research Articles on ATG14

1. Cisplatin upregulated Barkor protein levels of the Saos-2 cell line.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ATG14 antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-ATG14 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >4 publications with ATG14 and Kidney Diseases
 Disease Models, Animal Antibodies  >3 publications with ATG14 and Disease Models, Animal
 Nervous System Diseases Antibodies  >3 publications with ATG14 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with ATG14 and Brain Diseases
 Inflammation Antibodies  >1 publications with ATG14 and Inflammation
 Atrophy Antibodies  >1 publications with ATG14 and Atrophy
 Necrosis Antibodies  >1 publications with ATG14 and Necrosis

Organs/Tissues associated with anti-ATG14 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >8 publications with ATG14 and Kidney
 Liver Antibodies  >2 publications with ATG14 and Liver
 Embryonic Tissue Antibodies  >1 publications with ATG14 and Embryonic Tissue
 Intestine Antibodies  >1 publications with ATG14 and Intestine
 Heart Antibodies  >1 publications with ATG14 and Heart
 Muscle Antibodies  >1 publications with ATG14 and Muscle
 Brain Antibodies  >1 publications with ATG14 and Brain
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