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LTA4H Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249061

MBS249061 is an antibody targeting LTA4H. Also known as Mouse Monoclonal [clone 9G8] (IgG2a) to Human LTA4H / LTA4. Reactive in Human. Host: Mouse. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Product Name
LTA4H / LTA4, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 9G8] (IgG2a) to Human LTA4H / LTA4
Product Gene Name
Product Synonym Gene Name
LTA4
Product Synonym Names
Anti-LTA4H / LTA4 Antibody (clone 9G8) IHC-plus; LTA4H; Leukotriene A(4) hydrolase; Leukotriene A4 hydrolase; Leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4; Human LTA4H; LTA4
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9G8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
0.91 mg/ml (lot specific)
Specificity
Human LTA4H / LTA4
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
66,852 Da
OMIM
151570
3D Structure
ModBase 3D Structure for P09960
Target Species
Human
Immunogen Description
Full length human recombinant protein of human LTA4H (NP_000886) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
LTA4H / LTA4 antibody was raised against full length human recombinant protein of human LTA4H (NP_000886) produced in HEK293T cell.
Applications Tested/Suitable for anti-LTA4H antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-LTA4H antibody
Flo (1:100), IF (1:100), IHC-P (10 ug/ml), WB (1:2000)
Usage: Concentration: 0.5-1.0 mg/ml (Lot dependent)

Immunohistochemistry (IHC) of anti-LTA4H antibody

Anti-LTA4H / LTA4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-LTA4H Monoclonal Antibody – Cat# MBS249061

Immunohistochemistry (IHC) of anti-LTA4H antibody

Anti-LTA4H / LTA4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-LTA4H Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249061

Immunofluorescence (IF) of anti-LTA4H antibody

Anti-LTA4H mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LTA4H.
Mouse Anti-LTA4H Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249061

Flow Cytometry (FC/FACS) of anti-LTA4H antibody

HEK293T cells transfected with either pCMV6-ENTRY LTA4H (Red) or empty vector control plasmid (Blue) were immunostained with anti-LTA4H mouse monoclonal, and then analyzed by flow cytometry.
Mouse Anti-LTA4H Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249061

Western Blot (WB) of anti-LTA4H antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H.
Mouse Anti-LTA4H Monoclonal Antibody, Western Blot (WB) – Cat# MBS249061

Other Notes

Small volumes of anti-LTA4H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LTA4H. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leukotriene A-4 hydrolase isoform 1
NCBI Official Synonym Full Names
leukotriene A4 hydrolase
NCBI Official Symbol
LTA4H
UniProt Protein Name
Leukotriene A-4 hydrolase
UniProt Synonym Protein Names
Leukotriene A(4) hydrolase
UniProt Gene Name
LTA4H
UniProt Synonym Gene Names
LTA4; LTA-4 hydrolase
UniProt Entry Name
LKHA4_HUMAN
NCBI Protein Information
leukotriene A-4 hydrolase; LTA-4 hydrolase

NCBI Summary for LTA4H

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for LTA4H

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.3.2.6; Hydrolase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; metallopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; inflammatory response; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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