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CD22 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249124

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked… More details

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Product Overview

Online Datasheet in PDF
Product Name
CD22, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1B1] (IgG1) to Human CD22
Product Gene Name
anti-CD22 antibody
Product Synonym Names
Anti-CD22 Antibody (clone 1B1) IHC-plus; CD22; CD22 molecule; CD22 antigen; SIGLEC2; SIGLEC-2; T-cell surface antigen Leu-14; B-cell receptor CD22; BL-CAM; Human CD22
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human CD22
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
OMIM
107266
3D Structure
ModBase 3D Structure for P20273
Target Species
Human
Immunogen Description
Full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.
Antigen type
Recombinant Protein
Immunogen Type
Recombinant protein
Immunogen
CD22 antibody was raised against full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.

Product Categories/Family for anti-CD22 antibody

Family: Immunoglobulin
Applications Tested/Suitable for anti-CD22 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CD22 antibody
IF (1:100), IHC-P (5 ug/ml), WB (1:4000)

Immunohistochemistry (IHC) of anti-CD22 antibody

Anti-CD22 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CD22 Monoclonal Antibody – Cat# MBS249124

Immunofluorescence (IF) of anti-CD22 antibody

Anti-CD22 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD22.
Mouse Anti-CD22 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249124

Western Blot (WB) of anti-CD22 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
Mouse Anti-CD22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249124

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD22 purchase

MBS249124 is an antibody targeting CD22. Also known as Mouse Monoclonal [clone 1B1] (IgG1) to Human CD22. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #
157168355
NCBI GeneID
933
NCBI Accession #
NP_001762.2
UniProt Primary Accession #
P20273
UniProt Secondary Accession #
O95699; O95701; O95702; O95703; Q01665; Q32M46; Q92872; Q92873; Q9UQA6; F5GYU4; F5H7U3
UniProt Related Accession #
P20273
GenBank Nucleotide #
NM_001771.3
NCBI Official Full Name
B-cell receptor CD22 isoform 1
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
CD22
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family
B-cell receptor
UniProt Gene Name
CD22
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_HUMAN
NCBI Protein Information
B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2

UniProt Comments for CD22

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: cell adhesion
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Research Articles on CD22

1. study detected the expression of CD22 and CD72 on B cells of myasthenia gravis, compared to multiple sclerosis patient controls and healthy controls y

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD22 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD22 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >958 publications with CD22 and Immune System Diseases
 Neoplasms, Experimental Antibodies  >105 publications with CD22 and Neoplasms, Experimental
 Death Antibodies  >63 publications with CD22 and Death
 Inflammation Antibodies  >56 publications with CD22 and Inflammation
 Necrosis Antibodies  >51 publications with CD22 and Necrosis
 Respiratory Tract Diseases Antibodies  >46 publications with CD22 and Respiratory Tract Diseases
 Cell Transformation, Neoplastic Antibodies  >23 publications with CD22 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >22 publications with CD22 and Kidney Diseases
 Liver Diseases Antibodies  >19 publications with CD22 and Liver Diseases
 Lung Diseases Antibodies  >18 publications with CD22 and Lung Diseases

Organs/Tissues associated with anti-CD22 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1051 publications with CD22 and Blood
 Bone Marrow Antibodies  >289 publications with CD22 and Bone Marrow
 Spleen Antibodies  >122 publications with CD22 and Spleen
 Lymph Node Antibodies  >111 publications with CD22 and Lymph Node
 Lymph Antibodies  >101 publications with CD22 and Lymph
 Skin Antibodies  >47 publications with CD22 and Skin
 Lung Antibodies  >32 publications with CD22 and Lung
 Thymus Antibodies  >31 publications with CD22 and Thymus
 Tonsil Antibodies  >31 publications with CD22 and Tonsil
 Kidney Antibodies  >31 publications with CD22 and Kidney
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