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FN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249466

FN1 Antibody, CIG Antibody, Cold-insoluble globulin Antibody, Fibronectin 1 Antibody, FINC Antibody, LETS Antibody, GFND Antibody, ED-B Antibody, Fibronectin Antibody, FN Antibody, FNZ Antibody, GFND2 Antibody, Migration-stimulating factor Antibody, MSF… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
FN1 / Fibronectin, Monoclonal Antibody
Full Product Name
Anti-FN1 / Fibronectin Antibody (clone 2F4) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 2F4] (IgG1) to Human FN1 / Fibronectin; Human FN1 / Fibronectin; CIG; Cold-insoluble globulin; Fibronectin 1; FINC; LETS; GFND; ED-B; Fibronectin; FN; FNZ; GFND2; Migration-stimulating factor; MSF
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2F4
Host
Mouse
Species Reactivity
Human
Form/Format
Ascites, 0.03% sodium azide
Purity/Purification
Ascites
Specificity
Human fibronectin
Preparation and Storage
Long term: -20 degree C
Short term: 4 degree C
Avoid freeze-thaw cycles.
Molecular Weight
256,502 Da
OMIM
135600
3D Structure
ModBase 3D Structure for P02751
Immunogen
FN1 / Fibronectin antibody was raised against purified recombinant fragment of human FN1 expressed in E Coli.
Immunogen Description
Purified recombinant fragment of human FN1 expressed in E Coli.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-FN1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-FN1 antibody
ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
IHC-P (1:50)
WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-FN1 antibody

Anti-FN1 / Fibronectin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-FN1 Monoclonal Antibody – Cat# MBS249466

Immunohistochemistry (IHC) of anti-FN1 antibody

Anti-FN1 / Fibronectin antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-FN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249466

Western Blot (WB) of anti-FN1 antibody

Western blot using FN1 monoclonal antibody against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa)
Mouse Anti-FN1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249466

Western Blot (WB) of anti-FN1 antibody

Western blot using FN1 monoclonal antibody against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Mouse Anti-FN1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249466

ELISA (EIA) of anti-FN1 antibody

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Mouse Anti-FN1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS249466

Other Notes

Small volumes of anti-FN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FN1 purchase

MBS249466 is an antibody targeting FN1. Also known as Anti-FN1 / Fibronectin Antibody (clone 2F4) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fibronectin isoform 3 preproprotein
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
UniProt Protein Name
Fibronectin
UniProt Synonym Protein Names
Cold-insoluble globulin; CIG
Protein Family
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN; FN; CIG
UniProt Entry Name
FINC_HUMAN
NCBI Protein Information
fibronectin

NCBI Summary for FN1

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
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UniProt Comments for FN1

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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