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PARPBP / C12ORF48 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249625

PARPBP Antibody, AROM Antibody, C12orf48 Antibody, PARP1-binding protein Antibody, PARI Antibody, PARP-1 binding protein Antibody, PARP1 binding protein Antibody, PCNA-interacting partner Antibody Description: Required to suppress inappropriate homologous… More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PARPBP / C12orf48, Polyclonal Antibody
Full Product Name
Anti-PARPBP / C12orf48 Antibody (aa522-550) IHC-plus
Product Gene Name
anti-PARPBP / C12orf48 antibody
Product Synonym Names
Rabbit Polyclonal to Human PARPBP / C12orf48; Human PARPBP / C12orf48; AROM; PARP1-binding protein; PARI; PARP-1 binding protein; PARP1 binding protein; PCNA-interacting partner
Clonality
Polyclonal
Host
PARPBP/C12orf48 antibody was produced in Rabbit.
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein A purified
Specificity
This C12orf48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 522-550 amino acids from the C-terminal region of human C12orf48.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 6 months.
For long term storage store at -20°C.
OMIM
613687
3D Structure
ModBase 3D Structure for Q9NWS1
Immunogen
PARPBP / C12orf48 antibody was raised against Human.
Epitope
aa522-550
Note
During shipment, small volumes of product will occasionally become entraped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-PARPBP / C12orf48 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PARPBP / C12orf48 antibody
IHC-P (1:50 - 1:100)
WB (1:1000)
Flow Cytometry (1:10 - 1:50)

(Optimal dilution to be determined by the researcher.

Immunohistochemistry - Paraffin (IHC) of anti-PARPBP / C12orf48 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-PARPBP / C12ORF48 Polyclonal Antibody – Cat# MBS249625

Western Blot (WB) of anti-PARPBP / C12orf48 antibody

C12orf48 Antibody western blot of A549 cell line lysates (35 ug/lane). The C12orf48 antibody detected the FBXO34 protein (arrow).
Rabbit Anti-PARPBP / C12ORF48 Polyclonal Antibody, Western Blot (WB) – Cat# MBS249625

Flow Cytometry (FC/FACS) of anti-PARPBP / C12orf48 antibody

C12orf48 Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PARPBP / C12ORF48 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249625

Other Notes

Small volumes of anti-PARPBP / C12orf48 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PARPBP / C12orf48 purchase

MBS249625 is an antibody targeting PARPBP / C12orf48. Also known as Anti-PARPBP / C12orf48 Antibody (aa522-550) IHC-plus. Reactive in Human. Host: PARPBP/C12orf48 antibody was produced in Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PARPBP / C12orf48. It may not necessarily be applicable to this product.

NCBI GI #
300797388
NCBI GeneID
55010
NCBI Accession #
NP_060385.3
UniProt Primary Accession #
Q9NWS1
UniProt Secondary Accession #
Q05C00; Q499L8; Q4FZA0; Q4KMW7; Q6NSC6; Q6PJA1; Q86W36; B4E0Y0
UniProt Related Accession #
Q9NWS1
GenBank Nucleotide #
NM_017915.4
NCBI Official Full Name
PCNA-interacting partner isoform 2
NCBI Official Synonym Full Names
PARP1 binding protein
NCBI Official Symbol
PARPBP
NCBI Official Synonym Symbols
AROM; PARI; C12orf48
UniProt Protein Name
PCNA-interacting partner
UniProt Synonym Protein Names
PARP-1 binding protein; PARP1-binding protein; PARPBP
UniProt Gene Name
PARPBP
UniProt Synonym Gene Names
C12orf48; PARI; PARI; PARPBP
UniProt Entry Name
PARI_HUMAN
NCBI Protein Information
PCNA-interacting partner

UniProt Comments for PARPBP / C12orf48

PARPBP: Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP- ribosyl)ation activity of PARP1; however such function may be indirect. Belongs to the PARI family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: chromatin; cytoplasm; nucleoplasm

Molecular Function: DNA binding; protein binding

Biological Process: DNA repair
Show more

Research Articles on PARPBP / C12orf48

1. PARI overexpression promotes genomic instability and pancreatic tumorigenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PARPBP / C12orf48 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with PARPBP / C12orf48 and Cell Transformation, Neoplastic
 Hypertrophy Antibodies  >1 publications with PARPBP / C12orf48 and Hypertrophy

Organs/Tissues associated with anti-PARPBP / C12orf48 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >1 publications with PARPBP / C12orf48 and Prostate
 Brain Antibodies  >1 publications with PARPBP / C12orf48 and Brain
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