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F11 Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249882

F11 Antibody, Coagulation factor XI Antibody, Factor xi deficiency Antibody, F11 deficiency Antibody, Factor XI Antibody, PTA deficiency Antibody, PTA Antibody, Rosenthal syndrome Antibody, FXI Antibody Description: F11 / FXI / Factor XI is coagulation factor… More details

$680 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F11 / FXI / Factor XI, Polyclonal Antibody
Full Product Name
Anti-F11 / FXI / Factor XI Antibody IHC-plus
Product Gene Name
anti-F11 antibody
Product Synonym Names
Sheep Polyclonal (IgG) to Human F11 / FXI / Factor XI; Human F11 / FXI / Factor XI; Coagulation factor XI; Factor xi deficiency; F11 deficiency; Factor XI; PTA deficiency; PTA; Rosenthal syndrome; FXI
Clonality
Polyclonal
Isotype
IgG
Host
F11/ FX1/Factor XI antibody was produced in Sheep.
Species Reactivity
Human
Form/Format
ddH20, 50% glycerol.
Purity/Purification
Purified
Concentration
1 mg/ml (lot specific)
Specificity
Recognizes human Factor XI.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20° C. Aliquots are stable for at least 12 months.
OMIM
264900
3D Structure
ModBase 3D Structure for P03951
Family/Subfamily
Protease/Serine S1
Immunogen Species
F11/FX1/Factor XI antibody was raised against Human
Immunogen
F11/FX1/Factor XI antibody was raised against purified human Factor XI.
Note
During shipment,small volumes of profuct will occasionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
Warning
This reagent may contain sodium azide .The chemical,physical and toxicological properties of thsi material have not been thoroughly investigated. Standard Laboratory practices should be followed. Avoid skin and eye contact, inhalation and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent acumulations.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-F11 antibody
ELISA, Western Blot
Application Notes for anti-F11 antibody
IHC-Paraffin (5 ug/mL)
Western Blot (10 ug/mL)
ELISA (10 ug/mL)

Optimal dilution to be determined by the researcher.

Other Notes

Small volumes of anti-F11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F11 purchase

MBS249882 is an antibody targeting F11. Also known as Anti-F11 / FXI / Factor XI Antibody IHC-plus. Reactive in Human. Host: F11/ FX1/Factor XI antibody was produced in Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for F11. It may not necessarily be applicable to this product.

NCBI GI #
4503627
NCBI GeneID
2160
NCBI Accession #
NP_000119.1
UniProt Primary Accession #
P03951
UniProt Secondary Accession #
Q4W5C2; Q9Y495; D3DP64
UniProt Related Accession #
P03951
GenBank Nucleotide #
NM_000128.3
NCBI Official Full Name
coagulation factor XI preproprotein
NCBI Official Synonym Full Names
coagulation factor XI
NCBI Official Symbol
F11
NCBI Official Synonym Symbols
FXI
UniProt Protein Name
Coagulation factor XI
UniProt Synonym Protein Names
Plasma thromboplastin antecedent; PTA
Protein Family
Coagulation factor
UniProt Gene Name
F11
UniProt Synonym Gene Names
FXI; PTA
UniProt Entry Name
FA11_HUMAN
NCBI Protein Information
coagulation factor XI

NCBI Summary for F11

This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq, Jul 2008]
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UniProt Comments for F11

F11: Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. Defects in F11 are the cause of factor XI deficiency (FA11D); also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Belongs to the peptidase S1 family. Plasma kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.27; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular region; extracellular space; membrane; plasma membrane

Molecular Function: heparin binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; plasminogen activation; positive regulation of fibrinolysis

Disease: Factor Xi Deficiency
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Research Articles on F11

1. In whites, the FXI variant was associated with both factor XI concentration and venous thromboembolism (VTE) incidence (1.15-fold greater incidence of VTE per risk allele), whereas In African-Americans, these associations were absent.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-F11 antibody

 Reference Product  PubMed Publications
 F12 antibody  >35 publications with F11 and F12
 KNG1 antibody  >7 publications with F11 and KNG1
 APP antibody  >6 publications with F11 and APP
 SERPINC1 antibody  >6 publications with F11 and SERPINC1

Pathways associated with anti-F11 antibody

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway Of Fibrin Clot Formation antibodies  Intrinsic Pathway Of Fibrin Clot Formation Diagram

Diseases associated with anti-F11 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >62 publications with F11 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >49 publications with F11 and Cardiovascular Diseases
 Brain Diseases Antibodies  >37 publications with F11 and Brain Diseases
 Factor XI Deficiency Antibodies  >34 publications with F11 and Factor XI Deficiency
 Inflammation Antibodies  >24 publications with F11 and Inflammation
 Poisoning Antibodies  >14 publications with F11 and Poisoning
 Hypertension Antibodies  >12 publications with F11 and Hypertension
 Heart Diseases Antibodies  >11 publications with F11 and Heart Diseases
 Neurotoxicity Syndromes Antibodies  >11 publications with F11 and Neurotoxicity Syndromes
 Neoplasms, Experimental Antibodies  >9 publications with F11 and Neoplasms, Experimental

Organs/Tissues associated with anti-F11 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >37 publications with F11 and Kidney
 Eye Antibodies  >21 publications with F11 and Eye
 Liver Antibodies  >19 publications with F11 and Liver
 Lung Antibodies  >18 publications with F11 and Lung
 Intestine Antibodies  >15 publications with F11 and Intestine
 Prostate Antibodies  >11 publications with F11 and Prostate
 Pancreas Antibodies  >9 publications with F11 and Pancreas
 Bladder Antibodies  >6 publications with F11 and Bladder
 Cervix Antibodies  >1 publications with F11 and Cervix
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