Resolvin E1 (RvE1) ELISA Kit for Human

Principle of the Assay: RvE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RvE1 antibody and an RvE1-HRP conjugate. The assay sample and buffer are incubated together with RvE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RvE1 concentration since RvE1 from samples and RvE1-HRP conjugate compete for the anti-RvE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RvE1 from the sample, fewer sites are left to bind RvE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RvE1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This RvE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RvE1. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of RvE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS744335 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Resolvin E1 (RvE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RvE1. The ELISA analytical biochemical technique of the MBS744335 kit is based on RvE1 antibody-RvE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RvE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RvE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Due to recently published contradictory data, the need to further the understanding of both the synthesis and functions of human resolvin (RVE) has emerged. Resolvin E1 RVE1 is a family member of RVD and it's role in mediating PMN cell-based inflammation is well documented and derivation from fatty acids EPA well characterized, less well studied is it's anti inflammatory role in acute lung injury and pneumonia. To answer at least some of these experimental questions in cells or aspiration products, MyBiosource offers a competitive ELISA assay system Promega, to detect Rve1 Rvd1 protein. Rat Resolvin E1 has been previously studied (free full text PMC free article (s) thru PubMed) to gain an understanding of PMN transmigration, endothelial cells response and leukocytes ( PMNS) cell proliferation to injury, as models of crit care med. Our competitive RVE1 ELISA kit product will fill the science void for analysis of human RVE E1.