Protein Kinase B Gamma (PKBG) ELISA Kit for Cavy

Name: Cavy PKBG ELISA Kit; Protein Kinase B Gamma; MyBioSource
Brand: MyBioSource
SKU: MBS027988
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS027988

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PKBG concentrations in Cavy serum, plasma and other body fluids. Using Purified Cavy PKBG antibody to coat Microelisa Stripplate wells… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protein Kinase B Gamma (PKBG), ELISA Kit

Full Product Name

Cavy Protein Kinase B Gamma ELISA Kit

Product Gene Name

PKBG elisa kit

Species Reactivity

Cavy

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

3.12ng/ml-100ng/ml.

Sensitivity

1.0ng/ml.

Specificity

No significant cross-reactivity or interference between Cavy PKBG and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

55,775 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (243651535..244006886, complement). Location: 1q44

OMIM

603387

3D Structure

ModBase 3D Structure for Q9Y243

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PKBG concentrations in Cavy serum, plasma and other body fluids. Using Purified Cavy PKBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PKBG and PKBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PKBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of PKBG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKBG purchase

MBS027988 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Kinase B Gamma (PKBG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PKBG. The ELISA analytical biochemical technique of the MBS027988 kit is based on PKBG antibody-PKBG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKBG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKBG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PKBG. It may not necessarily be applicable to this product.

NCBI GI #

4757579

NCBI GeneID

10000

NCBI Accession #

AAD29089.1

UniProt Primary Accession #

Q9Y243

UniProt Secondary Accession #

Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5

UniProt Related Accession #

Q9Y243

NCBI Official Full Name

protein kinase B gamma

NCBI Official Synonym Full Names

v-akt murine thymoma viral oncogene homolog 3

NCBI Official Symbol

AKT3

NCBI Official Synonym Symbols

MPPH; PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma

UniProt Protein Name

RAC-gamma serine/threonine-protein kinase

UniProt Synonym Protein Names

Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2

Protein Family

RAC-gamma serine/threonine-protein kinase

UniProt Gene Name

AKT3

UniProt Synonym Gene Names

PKBG; PKB gamma

UniProt Entry Name

AKT3_HUMAN

NCBI Protein Information

RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

NCBI Summary for PKBG

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

UniProt Comments for PKBG

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; AKT family

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2

Research Articles on PKBG

1. AKT3, ANGPTL4, eNOS3, and VEGFA associations with high altitude sickness in Han and Tibetan Chinese at the Qinghai-Tibetan Plateau.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PKBG elisa kit

Reference Product	PubMed Publications
PTEN elisa kit	>50 publications with PKBG and PTEN
MTOR elisa kit	>27 publications with PKBG and MTOR
PIK3CA elisa kit	>20 publications with PKBG and PIK3CA
VEGFA elisa kit	>4 publications with PKBG and VEGFA
PDPK1 elisa kit	>2 publications with PKBG and PDPK1
TSC2 elisa kit	>2 publications with PKBG and TSC2
FLT1 elisa kit	>1 publications with PKBG and FLT1

Pathways associated with PKBG elisa kit

Products by Pathway	Pathway Diagram
AKT Phosphorylates Targets In The Cytosol Pathway antibodies	AKT Phosphorylates Targets In The Cytosol Pathway Diagram
AKT Phosphorylates Targets In The Nucleus Pathway antibodies	AKT Phosphorylates Targets In The Nucleus Pathway Diagram
AKT-mediated Inactivation Of FOXO1A Pathway antibodies	AKT-mediated Inactivation Of FOXO1A Pathway Diagram
Activation Of BAD And Translocation To Mitochondria Pathway antibodies	Activation Of BAD And Translocation To Mitochondria Pathway Diagram
Activation Of BH3-only Proteins Pathway antibodies	Activation Of BH3-only Proteins Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram

Diseases associated with PKBG elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>130 publications with PKBG and Neoplasms
Breast Neoplasms Antibodies	>32 publications with PKBG and Breast Neoplasms
Nervous System Diseases Antibodies	>29 publications with PKBG and Nervous System Diseases
Brain Diseases Antibodies	>18 publications with PKBG and Brain Diseases
Ovarian Neoplasms Antibodies	>16 publications with PKBG and Ovarian Neoplasms
Congenital Abnormalities Antibodies	>15 publications with PKBG and Congenital Abnormalities
Skin Neoplasms Antibodies	>14 publications with PKBG and Skin Neoplasms
Lung Neoplasms Antibodies	>12 publications with PKBG and Lung Neoplasms
Gastrointestinal Diseases Antibodies	>11 publications with PKBG and Gastrointestinal Diseases
Necrosis Antibodies	>8 publications with PKBG and Necrosis

Organs/Tissues associated with PKBG elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>39 publications with PKBG and Blood
Brain Antibodies	>39 publications with PKBG and Brain
Muscle Antibodies	>30 publications with PKBG and Muscle
Vascular Antibodies	>26 publications with PKBG and Vascular
Skin Antibodies	>22 publications with PKBG and Skin
Prostate Antibodies	>15 publications with PKBG and Prostate
Lung Antibodies	>15 publications with PKBG and Lung
Bone Antibodies	>12 publications with PKBG and Bone
Liver Antibodies	>11 publications with PKBG and Liver
Embryonic Tissue Antibodies	>9 publications with PKBG and Embryonic Tissue