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5-Hydroxytryptamine Receptor 2b (HTR2B) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280442

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR2B in samples. An antibody specific for HTR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHTR2B present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
5-hydroxytryptamine receptor 2B (HTR2B), ELISA Kit
Full Product Name
Rat 5-hydroxytryptamine receptor 2B (HTR2B) ELISA Kit
Product Gene Name
HTR2B elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat HTR2B. No significant cross-reactivity or interference between Rat HTR2B and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
53,652 Da
3D Structure
ModBase 3D Structure for P30994
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HTR2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HTR2B purchase

MBS280442 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 2B (HTR2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR2B. The ELISA analytical biochemical technique of the MBS280442 kit is based on HTR2B antibody-HTR2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HTR2B. It may not necessarily be applicable to this product.

NCBI GI #
8393586
NCBI GeneID
29581
NCBI Accession #
NP_058946.1
UniProt Primary Accession #
P30994
UniProt Secondary Accession #
Q9QW44
UniProt Related Accession #
P30994
GenBank Nucleotide #
NM_017250.1
NCBI Official Full Name
5-hydroxytryptamine receptor 2B
NCBI Official Synonym Full Names
5-hydroxytryptamine receptor 2B
NCBI Official Symbol
Htr2b
UniProt Protein Name
5-hydroxytryptamine receptor 2B
UniProt Synonym Protein Names
5-HT-2F
Protein Family
5-hydroxytryptamine receptor
UniProt Gene Name
Htr2b
UniProt Synonym Gene Names
Srl; 5-HT-2B; 5-HT2B
NCBI Protein Information
5-hydroxytryptamine receptor 2B

NCBI Summary for HTR2B

G-protein coupled receptor for serotonin [RGD, Feb 2006]

UniProt Comments for HTR2B

5-HT(2B): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 9q35

Cellular Component: cell junction; cell soma; cytoplasm; dendrite; integral component of plasma membrane; membrane; plasma membrane; synapse

Molecular Function: drug binding; G-protein alpha-subunit binding; G-protein coupled serotonin receptor activity; GTPase activator activity; neurotransmitter receptor activity; protein binding; serotonin binding

Biological Process: activation of phospholipase C activity; calcium-mediated signaling; cellular calcium ion homeostasis; cGMP biosynthetic process; embryonic morphogenesis; G-protein coupled receptor internalization; G-protein coupled receptor signaling pathway; heart development; heart morphogenesis; inositol phosphate metabolic process; intestine smooth muscle contraction; negative regulation of apoptosis; negative regulation of autophagy; neural crest cell differentiation; neural crest cell migration; phosphoinositide 3-kinase cascade; phospholipase C-activating G-protein coupled receptor signaling pathway; phosphorylation; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of endothelial cell proliferation; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of MAP kinase activity; positive regulation of nitric-oxide synthase activity; positive regulation of phosphatidylinositol biosynthetic process; protein kinase C-activating G-protein coupled receptor signaling pathway; regulation of behavior; release of sequestered calcium ion into cytosol; response to drug; serotonin receptor signaling pathway; vasoconstriction
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Research Articles on HTR2B

1. 5-HT stimulates DNA synthesis and proliferation in primary cultures of adult rat hepatocytes by acting via autocrine secretion of TGF-alpha induced by the serotonin 5-HT2B receptor/Gq/PLC/Ca2+ pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HTR2B elisa kit

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram

Diseases associated with HTR2B elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with HTR2B and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with HTR2B and Brain Diseases
 Obesity Antibodies  >3 publications with HTR2B and Obesity
 Mental Disorders Antibodies  >3 publications with HTR2B and Mental Disorders
 Serotonin Syndrome Antibodies  >2 publications with HTR2B and Serotonin Syndrome
 Neurologic Manifestations Antibodies  >1 publications with HTR2B and Neurologic Manifestations
 Liver Diseases Antibodies  >1 publications with HTR2B and Liver Diseases
 Migraine Disorders Antibodies  >1 publications with HTR2B and Migraine Disorders
 Obsessive-Compulsive Disorder Antibodies  >1 publications with HTR2B and Obsessive-Compulsive Disorder
 Anxiety Disorders Antibodies  >1 publications with HTR2B and Anxiety Disorders
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