Zinc Finger Matrin-Type Protein 5 (ZMAT5) ELISA Kit for Mouse

ZMAT5 Contains 1 C3H1-type zinc finger. Alternatively spliced transcript variants have been described. Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression.
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules. The name "zinc finger" was originally coined to describe the hypothesized structure of the repeated unit in Xenopus laevis transcription factor IIIA.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMAT5 in samples. An antibody specific for ZMAT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMAT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMAT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMAT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZMAT5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280587 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger matrin-type protein 5 (ZMAT5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZMAT5. The ELISA analytical biochemical technique of the MBS280587 kit is based on ZMAT5 antibody-ZMAT5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZMAT5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZMAT5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).