Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit for Rat

Name: Rat VCAM-1 ELISA Kit; Vascular Cell Adhesion Molecule 1; MyBioSource
Brand: MyBioSource
SKU: MBS280681
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280681

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VCAM1 in samples. An antibody specific for VCAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVCAM1 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Vascular cell adhesion molecule 1 (VCAM-1), ELISA Kit

Full Product Name

Rat Vascular cell adhesion molecule 1 (VCAM-1) ELISA Kit

Product Gene Name

VCAM-1 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat VCAM1. No significant cross-reactivity or interference between Rat VCAM1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

81,246 Da

3D Structure

ModBase 3D Structure for P29534

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of VCAM-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCAM-1 purchase

MBS280681 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular cell adhesion molecule 1 (VCAM-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCAM-1. The ELISA analytical biochemical technique of the MBS280681 kit is based on VCAM-1 antibody-VCAM-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for VCAM-1. It may not necessarily be applicable to this product.

NCBI GI #

6981700

NCBI GeneID

25361

NCBI Accession #

NP_037021.1

UniProt Primary Accession #

P29534

UniProt Secondary Accession #

Q5FVS3; Q63669

UniProt Related Accession #

P29534

GenBank Nucleotide #

NM_012889.1

NCBI Official Full Name

vascular cell adhesion protein 1

NCBI Official Synonym Full Names

vascular cell adhesion molecule 1

NCBI Official Symbol

Vcam1

NCBI Official Synonym Symbols

VCAM1B

UniProt Protein Name

Vascular cell adhesion protein 1

UniProt Synonym Protein Names

CD_antigen: CD106

UniProt Gene Name

Vcam1

UniProt Synonym Gene Names

Vcam-1; V-CAM 1; VCAM-1

NCBI Protein Information

vascular cell adhesion protein 1

NCBI Summary for VCAM-1

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

UniProt Comments for VCAM-1

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q41

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; integral component of membrane; microvillus; podosome; sarcolemma

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: acute inflammatory response; aging; amine metabolic process; cell adhesion; cell-matrix adhesion; chronic inflammatory response; heart development; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; response to ethanol; response to hypoxia; response to ionizing radiation; response to lipopolysaccharide; response to nicotine; response to nutrient; response to zinc ion; viral process

Research Articles on VCAM-1

1. Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with VCAM-1 elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram

Diseases associated with VCAM-1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>56 publications with VCAM-1 and Inflammation
Cardiovascular Diseases Antibodies	>42 publications with VCAM-1 and Cardiovascular Diseases
Necrosis Antibodies	>39 publications with VCAM-1 and Necrosis
Neoplasms Antibodies	>33 publications with VCAM-1 and Neoplasms
Atherosclerosis Antibodies	>29 publications with VCAM-1 and Atherosclerosis
Kidney Diseases Antibodies	>10 publications with VCAM-1 and Kidney Diseases
Hypertension Antibodies	>10 publications with VCAM-1 and Hypertension
Heart Diseases Antibodies	>9 publications with VCAM-1 and Heart Diseases
Myocardial Ischemia Antibodies	>7 publications with VCAM-1 and Myocardial Ischemia
Myocardial Infarction Antibodies	>6 publications with VCAM-1 and Myocardial Infarction

Organs/Tissues associated with VCAM-1 elisa kit

Organ/Tissue Name	Pubmed Publications
Artery Antibodies	>21 publications with VCAM-1 and Artery
Embryonic Tissue Antibodies	>19 publications with VCAM-1 and Embryonic Tissue
Kidney Antibodies	>17 publications with VCAM-1 and Kidney
Brain Antibodies	>12 publications with VCAM-1 and Brain
Lung Antibodies	>7 publications with VCAM-1 and Lung
Adipose Tissue Antibodies	>4 publications with VCAM-1 and Adipose Tissue
Eye Antibodies	>3 publications with VCAM-1 and Eye
Spleen Antibodies	>3 publications with VCAM-1 and Spleen
Pancreas Antibodies	>3 publications with VCAM-1 and Pancreas
Prostate Antibodies	>2 publications with VCAM-1 and Prostate