Phosphoenolpyruvate Carboxykinase [GTP] (PCK2) ELISA Kit for Human

Name: Human PCK2 ELISA Kit; Phosphoenolpyruvate Carboxykinase [GTP]; MyBioSource
Brand: MyBioSource
SKU: MBS281601
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281601

Intended Uses: For the quantitative detection of Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) concentration in serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphoenolpyruvate carboxykinase [GTP] (PCK2), ELISA Kit

Full Product Name

Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit

Product Gene Name

PCK2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

3.12 ng/mL - 200 ng/mL

Sensitivity

1.04 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PCK2. No significant cross-reactivity or interference between Human PCK2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

47,564 Da

OMIM

X92720 mRNA

3D Structure

ModBase 3D Structure for Q16822

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PCK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCK2 purchase

MBS281601 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoenolpyruvate carboxykinase [GTP] (PCK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCK2. The ELISA analytical biochemical technique of the MBS281601 kit is based on PCK2 antibody-PCK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PCK2. It may not necessarily be applicable to this product.

NCBI GI #

614458223

NCBI GeneID

5106

NCBI Accession #

NP_001018083.2

UniProt Primary Accession #

Q16822

UniProt Secondary Accession #

O43253; Q86U01; Q9BV62

UniProt Related Accession #

Q16822

GenBank Nucleotide #

NM_001018073.2

NCBI Official Full Name

phosphoenolpyruvate carboxykinase

NCBI Official Synonym Full Names

phosphoenolpyruvate carboxykinase 2, mitochondrial

NCBI Official Symbol

PCK2

NCBI Official Synonym Symbols

PEPCK; PEPCK2; PEPCK-M

UniProt Protein Name

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Protein Family

Phosphoenolpyruvate carboxykinase

UniProt Gene Name

PCK2

UniProt Synonym Gene Names

PEPCK2; PEPCK-M

NCBI Protein Information

phosphoenolpyruvate carboxykinase [GTP], mitochondrial

NCBI Summary for PCK2

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

UniProt Comments for PCK2

PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 4.1.1.32; Kinase, other; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: GTP binding; metal ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding

Biological Process: gluconeogenesis; NADH oxidation; oxaloacetate metabolic process; positive regulation of insulin secretion; pyruvate metabolic process; response to lipopolysaccharide

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial

Research Articles on PCK2

1. Downregulation of PCK2 remodels tricarboxylic acid cycle in tumor-repopulating cells of melanoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PCK2 elisa kit

Reference Product	PubMed Publications
AKT1 elisa kit	>2 publications with PCK2 and AKT1
PGC elisa kit	>1 publications with PCK2 and PGC
INS elisa kit	>1 publications with PCK2 and INS

Pathways associated with PCK2 elisa kit

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
FoxO Signaling Pathway antibodies	FoxO Signaling Pathway Diagram

Diseases associated with PCK2 elisa kit

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>6 publications with PCK2 and Fatty Liver
Carcinoma, Hepatocellular Antibodies	>3 publications with PCK2 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>3 publications with PCK2 and Liver Diseases
Liver Neoplasms Antibodies	>3 publications with PCK2 and Liver Neoplasms
Hepatitis Antibodies	>2 publications with PCK2 and Hepatitis
Adenocarcinoma Antibodies	>2 publications with PCK2 and Adenocarcinoma
Disease Models, Animal Antibodies	>2 publications with PCK2 and Disease Models, Animal
Weight Loss Antibodies	>1 publications with PCK2 and Weight Loss
Inflammation Antibodies	>1 publications with PCK2 and Inflammation
Necrosis Antibodies	>1 publications with PCK2 and Necrosis

Organs/Tissues associated with PCK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>18 publications with PCK2 and Liver
Blood Antibodies	>12 publications with PCK2 and Blood
Adipose Tissue Antibodies	>9 publications with PCK2 and Adipose Tissue
Muscle Antibodies	>5 publications with PCK2 and Muscle
Connective Tissue Antibodies	>5 publications with PCK2 and Connective Tissue
Kidney Antibodies	>4 publications with PCK2 and Kidney
Embryonic Tissue Antibodies	>3 publications with PCK2 and Embryonic Tissue
Pancreas Antibodies	>3 publications with PCK2 and Pancreas
Heart Antibodies	>2 publications with PCK2 and Heart
Ovary Antibodies	>1 publications with PCK2 and Ovary