Collagenase 3 (MMP13) ELISA Kit for Horse

Name: Horse MMP13 ELISA Kit; Collagenase 3; MyBioSource
Brand: MyBioSource
SKU: MBS281617
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281617

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MMP13 in Horse serum, plasma, tissue homogenates. An antibody specific for MMP13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Collagenase 3 (MMP13), ELISA Kit

Full Product Name

Horse Collagenase 3 (MMP13) ELISA Kit

Product Gene Name

MMP13 elisa kit

Species Reactivity

Horse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.625 ng/mL - 40 ng/mL

Sensitivity

0.31 ng/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Horse MMP13. No significant cross-reactivity or interference between Horse MMP13 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

54,226 Da

3D Structure

ModBase 3D Structure for O18927

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MMP13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP13 purchase

MBS281617 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagenase 3 (MMP13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP13. The ELISA analytical biochemical technique of the MBS281617 kit is based on MMP13 antibody-MMP13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MMP13. It may not necessarily be applicable to this product.

NCBI GI #

126352506

NCBI GeneID

100009711

NCBI Accession #

NP_001075273.1

UniProt Primary Accession #

O18927

UniProt Related Accession #

O18927

GenBank Nucleotide #

NM_001081804.1

NCBI Official Full Name

collagenase 3

NCBI Official Synonym Full Names

matrix metallopeptidase 13

NCBI Official Symbol

MMP13

UniProt Protein Name

Collagenase 3

UniProt Synonym Protein Names

Matrix metalloproteinase-13; MMP-13

Protein Family

Collagenase

UniProt Gene Name

MMP13

UniProt Synonym Gene Names

MMP-13

NCBI Protein Information

collagenase 3

UniProt Comments for MMP13

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().

Research Articles on MMP13

1. The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP13 elisa kit

Reference Product	PubMed Publications
TNF elisa kit	>120 publications with MMP13 and TNF
RUNX2 elisa kit	>86 publications with MMP13 and RUNX2
TIMP1 elisa kit	>75 publications with MMP13 and TIMP1
FOS elisa kit	>44 publications with MMP13 and FOS
TIMP2 elisa kit	>32 publications with MMP13 and TIMP2
FGF2 elisa kit	>20 publications with MMP13 and FGF2
ETS1 elisa kit	>6 publications with MMP13 and ETS1
TP53 elisa kit	>5 publications with MMP13 and TP53
TNC elisa kit	>4 publications with MMP13 and TNC
ETV4 elisa kit	>1 publications with MMP13 and ETV4

Diseases associated with MMP13 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>261 publications with MMP13 and Inflammation
Disease Models, Animal Antibodies	>211 publications with MMP13 and Disease Models, Animal
Necrosis Antibodies	>185 publications with MMP13 and Necrosis
Fibrosis Antibodies	>120 publications with MMP13 and Fibrosis
Neoplasm Invasiveness Antibodies	>86 publications with MMP13 and Neoplasm Invasiveness
Liver Diseases Antibodies	>79 publications with MMP13 and Liver Diseases
Hypertrophy Antibodies	>78 publications with MMP13 and Hypertrophy
Breast Neoplasms Antibodies	>58 publications with MMP13 and Breast Neoplasms
Pain Antibodies	>39 publications with MMP13 and Pain
Heart Diseases Antibodies	>34 publications with MMP13 and Heart Diseases