Caspase-6 (CASP6) ELISA Kit for Mouse

Name: Mouse CASP6 ELISA Kit; Caspase-6; MyBioSource
Brand: MyBioSource
SKU: MBS281999
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281999

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CASP6 in samples. An antibody specific for CASP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCASP6 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Caspase-6 (CASP6), ELISA Kit

Full Product Name

Mouse Caspase-6 (CASP6) ELISA Kit

Product Gene Name

CASP6 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CASP6. No significant cross-reactivity or interference between Mouse CASP6 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

31,595 Da

3D Structure

ModBase 3D Structure for O08738

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CASP6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASP6 purchase

MBS281999 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase-6 (CASP6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CASP6. The ELISA analytical biochemical technique of the MBS281999 kit is based on CASP6 antibody-CASP6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASP6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASP6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CASP6. It may not necessarily be applicable to this product.

NCBI GI #

157951736

NCBI GeneID

12368

NCBI Accession #

NP_033941.3

UniProt Primary Accession #

O08738

UniProt Related Accession #

O08738

GenBank Nucleotide #

NM_009811.4

NCBI Official Full Name

caspase-6

NCBI Official Synonym Full Names

caspase 6

NCBI Official Symbol

Casp6

NCBI Official Synonym Symbols

Mch2; CASP-6

UniProt Protein Name

Caspase-6

UniProt Synonym Protein Names

Apoptotic protease Mch-2

Protein Family

Caspase

UniProt Gene Name

Casp6

UniProt Synonym Gene Names

CASP-6

NCBI Protein Information

caspase-6

NCBI Summary for CASP6

This gene encodes a member of the cysteine proteases that plays important roles in regulating apoptosis and neurodegeneration. The encoded protein is involved in the transmission of pain and axonal degeneration. Genetic deletion of this gene in mice results in the delay of axon pruning and protects from axon degeneration. [provided by RefSeq, Apr 2015]

UniProt Comments for CASP6

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.59; Protease

Chromosomal Location of Human Ortholog: 3|3 G3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; identical protein binding; peptidase activity; protein binding

Biological Process: acute inflammatory response to non-antigenic stimulus; apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptotic process; positive regulation of retinal cell programmed cell death; proteolysis; regulation of apoptosis; response to cytokine; response to glucose stimulus; response to hydrogen peroxide; response to iron ion

Research Articles on CASP6

1. Caspases and their substrates are key mediators of apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CASP6 elisa kit

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation By HSP70 Pathway antibodies	Apoptosis Modulation By HSP70 Pathway Diagram
Apoptosis Signaling Pathway antibodies	Apoptosis Signaling Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Breakdown Of The Nuclear Lamina Pathway antibodies	Breakdown Of The Nuclear Lamina Pathway Diagram
Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies	Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram

Diseases associated with CASP6 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>179 publications with CASP6 and Neoplasms
Necrosis Antibodies	>77 publications with CASP6 and Necrosis
Nervous System Diseases Antibodies	>72 publications with CASP6 and Nervous System Diseases
Drug Toxicity Antibodies	>41 publications with CASP6 and Drug Toxicity
Disease Models, Animal Antibodies	>34 publications with CASP6 and Disease Models, Animal
Breast Neoplasms Antibodies	>32 publications with CASP6 and Breast Neoplasms
Adenocarcinoma Antibodies	>27 publications with CASP6 and Adenocarcinoma
Inflammation Antibodies	>22 publications with CASP6 and Inflammation
Nerve Degeneration Antibodies	>20 publications with CASP6 and Nerve Degeneration
Cognition Disorders Antibodies	>18 publications with CASP6 and Cognition Disorders

Organs/Tissues associated with CASP6 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>71 publications with CASP6 and Brain
Liver Antibodies	>30 publications with CASP6 and Liver
Lung Antibodies	>26 publications with CASP6 and Lung
Skin Antibodies	>21 publications with CASP6 and Skin
Muscle Antibodies	>21 publications with CASP6 and Muscle
Kidney Antibodies	>21 publications with CASP6 and Kidney
Prostate Antibodies	>17 publications with CASP6 and Prostate
Embryonic Tissue Antibodies	>15 publications with CASP6 and Embryonic Tissue
Eye Antibodies	>14 publications with CASP6 and Eye
Bone Antibodies	>14 publications with CASP6 and Bone