Mouse ATP6V1H ELISA Kit – Cat# MBS282191

V-Type Proton Atpase Subunit H (ATP6V1H) ELISA Kit for Mouse

Name: Mouse ATP6V1H ELISA Kit; V-Type Proton Atpase Subunit H; MyBioSource
Brand: MyBioSource
SKU: MBS282191
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282191

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ATP6V1H in samples. An antibody specific for ATP6V1H has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATP6V1H present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

V-type proton ATPase subunit H (ATP6V1H), ELISA Kit

Full Product Name

Mouse V-type proton ATPase subunit H (ATP6V1H) ELISA Kit

Product Gene Name

ATP6V1H elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse ATP6V1H. No significant cross-reactivity or interference between Mouse ATP6V1H and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

55,855 Da

3D Structure

ModBase 3D Structure for Q8BVE3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ATP6V1H elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP6V1H purchase

MBS282191 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-type proton ATPase subunit H (ATP6V1H) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP6V1H. The ELISA analytical biochemical technique of the MBS282191 kit is based on ATP6V1H antibody-ATP6V1H antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP6V1H antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP6V1H. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ATP6V1H. It may not necessarily be applicable to this product.

NCBI GI #

882939074

NCBI GeneID

108664

NCBI Accession #

NP_001297371.1

UniProt Primary Accession #

Q8BVE3

UniProt Secondary Accession #

Q921X8

UniProt Related Accession #

Q8BVE3

GenBank Nucleotide #

NM_001310442.1

NCBI Official Full Name

V-type proton ATPase subunit H isoform 2

NCBI Official Synonym Full Names

ATPase, H+ transporting, lysosomal V1 subunit H

NCBI Official Symbol

Atp6v1h

NCBI Official Synonym Symbols

SFD; VMA13; CGI-11; SFDbeta; AU022349; SFDalpha; 0710001F19Rik

UniProt Protein Name

V-type proton ATPase subunit H

UniProt Synonym Protein Names

Vacuolar proton pump subunit H

Protein Family

V-type proton ATPase

UniProt Gene Name

Atp6v1h

UniProt Synonym Gene Names

V-ATPase subunit H

NCBI Protein Information

V-type proton ATPase subunit H

UniProt Comments for ATP6V1H

ATP6V1H: Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. Belongs to the V-ATPase H subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: 1|1 A1

Cellular Component: extracellular exosome; lysosomal membrane; vacuolar proton-transporting V-type ATPase, V1 domain

Molecular Function: hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: ATP hydrolysis coupled proton transport; endocytosis; ion transport; proton transport; transport; vacuolar acidification

Research Articles on ATP6V1H

1. Differences between two isoforms using bioinformatic methods, as well as some in vitro and in vivo methods in mice and zebrafish; found that two isoforms of H subunit showed the tissue specific locations and the ratio of two subunits might have an important role to control osteoclast function and craniofacial development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ATP6V1H elisa kit

Reference Product	PubMed Publications
Atp6v1b2 elisa kit	>1 publications with ATP6V1H and Atp6v1b2
Atp6v0d1 elisa kit	>1 publications with ATP6V1H and Atp6v0d1
Atp6v1f elisa kit	>1 publications with ATP6V1H and Atp6v1f
Atp6v1e1 elisa kit	>1 publications with ATP6V1H and Atp6v1e1

Pathways associated with ATP6V1H elisa kit

Products by Pathway	Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Insulin Receptor Recycling Pathway antibodies	Insulin Receptor Recycling Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Iron Uptake And Transport Pathway antibodies	Iron Uptake And Transport Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Oxidative Phosphorylation Pathway antibodies	Oxidative Phosphorylation Pathway Diagram
Oxidative Phosphorylation Pathway antibodies	Oxidative Phosphorylation Pathway Diagram

Diseases associated with ATP6V1H elisa kit

Disease Name	Pubmed Publications
Lung Diseases Antibodies	>1 publications with ATP6V1H and Lung Diseases
Insulin Resistance Antibodies	>1 publications with ATP6V1H and Insulin Resistance

Organs/Tissues associated with ATP6V1H elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>4 publications with ATP6V1H and Bone
Pancreas Antibodies	>2 publications with ATP6V1H and Pancreas
Blood Antibodies	>1 publications with ATP6V1H and Blood
Lung Antibodies	>1 publications with ATP6V1H and Lung
Eye Antibodies	>1 publications with ATP6V1H and Eye
Embryonic Tissue Antibodies	>1 publications with ATP6V1H and Embryonic Tissue