Pre-Mrna-Processing Factor 19 (PRPF19) ELISA Kit for Human

Name: Human PRPF19 ELISA Kit; Pre-Mrna-Processing Factor 19; MyBioSource
Brand: MyBioSource
SKU: MBS282212
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282212

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRPF19 in samples. An antibody specific for PRPF19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF19 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pre-mRNA-processing factor 19 (PRPF19), ELISA Kit

Full Product Name

Human Pre-mRNA-processing factor 19 (PRPF19) ELISA Kit

Product Gene Name

PRPF19 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PRPF19. No significant cross-reactivity or interference between Human PRPF19 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

55,181 Da

OMIM

AJ131186 mRNA

3D Structure

ModBase 3D Structure for Q9UMS4

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PRPF19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRPF19 purchase

MBS282212 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pre-mRNA-processing factor 19 (PRPF19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRPF19. The ELISA analytical biochemical technique of the MBS282212 kit is based on PRPF19 antibody-PRPF19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRPF19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRPF19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRPF19. It may not necessarily be applicable to this product.

NCBI GI #

7657381

NCBI GeneID

27339

NCBI Accession #

NP_055317.1

UniProt Primary Accession #

Q9UMS4

UniProt Related Accession #

Q9UMS4

GenBank Nucleotide #

NM_014502.4

NCBI Official Full Name

pre-mRNA-processing factor 19

NCBI Official Synonym Full Names

pre-mRNA processing factor 19

NCBI Official Symbol

PRPF19

NCBI Official Synonym Symbols

PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200

UniProt Protein Name

Pre-mRNA-processing factor 19

UniProt Synonym Protein Names

Nuclear matrix protein 200

Protein Family

Pre-mRNA-processing factor

UniProt Gene Name

PRPF19

UniProt Synonym Gene Names

hPso4

NCBI Protein Information

pre-mRNA-processing factor 19

NCBI Summary for PRPF19

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]

UniProt Comments for PRPF19

PRPF19: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the WD repeat PRP19 family.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; RNA splicing; Spliceosome; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: cytoplasm; DNA replication factor A complex; lipid droplet; membrane; nuclear speck; nucleoplasm; nucleus; spindle

Molecular Function: identical protein binding; protein binding

Biological Process: double-strand break repair via nonhomologous end joining; generation of catalytic spliceosome for first transesterification step; inner cell mass cell proliferation; lipid biosynthetic process; mRNA splicing, via spliceosome; negative regulation of neuron differentiation; positive regulation of astrocyte differentiation; positive regulation of mRNA splicing, via spliceosome; proteasomal protein catabolic process; protein polyubiquitination; spliceosomal complex assembly; spliceosomal tri-snRNP complex assembly; transcription-coupled nucleotide-excision repair

Research Articles on PRPF19

1. This work implies that the gain of Prp19 is a critical event during the progression of hepatocellular carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PRPF19 elisa kit

Products by Pathway	Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome, 35S U5-snRNP Pathway antibodies	Spliceosome, 35S U5-snRNP Pathway Diagram
Spliceosome, Prp19/CDC5L Complex Pathway antibodies	Spliceosome, Prp19/CDC5L Complex Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with PRPF19 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>2 publications with PRPF19 and Carcinoma
Cell Transformation, Neoplastic Antibodies	>1 publications with PRPF19 and Cell Transformation, Neoplastic
Pancreatic Neoplasms Antibodies	>1 publications with PRPF19 and Pancreatic Neoplasms
Disease Models, Animal Antibodies	>1 publications with PRPF19 and Disease Models, Animal
Genomic Instability Antibodies	>1 publications with PRPF19 and Genomic Instability
Carcinoma, Hepatocellular Antibodies	>1 publications with PRPF19 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with PRPF19 and Liver Neoplasms

Organs/Tissues associated with PRPF19 elisa kit

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>1 publications with PRPF19 and Nerve
Heart Antibodies	>1 publications with PRPF19 and Heart
Blood Antibodies	>1 publications with PRPF19 and Blood
Liver Antibodies	>1 publications with PRPF19 and Liver
Pancreas Antibodies	>1 publications with PRPF19 and Pancreas
Brain Antibodies	>1 publications with PRPF19 and Brain