Phosphatidylinositol-Glycan Biosynthesis Class W (PIGW) ELISA Kit for Human

Name: Human PIGW ELISA Kit; Phosphatidylinositol-Glycan Biosynthesis Class W; MyBioSource
Brand: MyBioSource
SKU: MBS282553
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282553

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIGW in samples. An antibody specific for PIGW has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGW present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphatidylinositol-glycan biosynthesis class W protein (PIGW), ELISA Kit

Full Product Name

Human Phosphatidylinositol-glycan biosynthesis class W protein (PIGW) ELISA Kit

Product Gene Name

PIGW elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PIGW. No significant cross-reactivity or interference between Human PIGW and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

56,882 Da

OMIM

AB097818 mRNA

3D Structure

ModBase 3D Structure for Q7Z7B1

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PIGW elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIGW purchase

MBS282553 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphatidylinositol-glycan biosynthesis class W protein (PIGW) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIGW. The ELISA analytical biochemical technique of the MBS282553 kit is based on PIGW antibody-PIGW antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIGW antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIGW. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PIGW. It may not necessarily be applicable to this product.

NCBI GI #

1092878785

NCBI GeneID

284098

NCBI Accession #

NP_001333683.1

UniProt Primary Accession #

Q7Z7B1

UniProt Secondary Accession #

Q8N9G3

UniProt Related Accession #

Q7Z7B1

GenBank Nucleotide #

NM_001346754.1

NCBI Official Full Name

phosphatidylinositol-glycan biosynthesis class W protein

NCBI Official Synonym Full Names

phosphatidylinositol glycan anchor biosynthesis class W

NCBI Official Symbol

PIGW

NCBI Official Synonym Symbols

Gwt1; HPMRS5

UniProt Protein Name

Phosphatidylinositol-glycan biosynthesis class W protein

Protein Family

Phosphatidylinositol-glycan biosynthesis class W protein

UniProt Gene Name

PIGW

UniProt Synonym Gene Names

PIG-W

NCBI Protein Information

phosphatidylinositol-glycan biosynthesis class W protein

NCBI Summary for PIGW

The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017]

UniProt Comments for PIGW

PIGW: Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI- anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins. Belongs to the PIGW family.

Protein type: EC 2.3.-.-; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: endoplasmic reticulum membrane; integral component of membrane

Molecular Function: O-acyltransferase activity

Biological Process: GPI anchor metabolic process; preassembly of GPI anchor in ER membrane

Disease: Hyperphosphatasia With Mental Retardation Syndrome 5

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PIGW elisa kit

Reference Product	PubMed Publications
PGAP1 elisa kit	>1 publications with PIGW and PGAP1
PIGN elisa kit	>1 publications with PIGW and PIGN
PIGO elisa kit	>1 publications with PIGW and PIGO
PIGG elisa kit	>1 publications with PIGW and PIGG
PIGL elisa kit	>1 publications with PIGW and PIGL

Pathways associated with PIGW elisa kit

Products by Pathway	Pathway Diagram
GPI-anchor Biosynthesis, Core Oligosaccharide Pathway antibodies	GPI-anchor Biosynthesis, Core Oligosaccharide Pathway Diagram
GPI-anchor Biosynthesis, Core Oligosaccharide Pathway antibodies	GPI-anchor Biosynthesis, Core Oligosaccharide Pathway Diagram
Glycosylphosphatidylinositol (GPI)-anchor Biosynthesis Pathway antibodies	Glycosylphosphatidylinositol (GPI)-anchor Biosynthesis Pathway Diagram
Glycosylphosphatidylinositol (GPI)-anchor Biosynthesis Pathway antibodies	Glycosylphosphatidylinositol (GPI)-anchor Biosynthesis Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway antibodies	Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Synthesis Of Glycosylphosphatidylinositol (GPI) Pathway antibodies	Synthesis Of Glycosylphosphatidylinositol (GPI) Pathway Diagram

Diseases associated with PIGW elisa kit

Disease Name	Pubmed Publications
Seizures Antibodies	>3 publications with PIGW and Seizures
Atrophy Antibodies	>2 publications with PIGW and Atrophy
Nervous System Diseases Antibodies	>2 publications with PIGW and Nervous System Diseases
Hyperphosphatasia with Mental Retardation Antibodies	>2 publications with PIGW and Hyperphosphatasia with Mental Retardation
Anemia Antibodies	>1 publications with PIGW and Anemia

Organs/Tissues associated with PIGW elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1 publications with PIGW and Liver
Brain Antibodies	>1 publications with PIGW and Brain