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Phosphoribosyltransferase Domain-Containing Protein 1 (PRTFDC1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282589

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRTFDC1 in samples. An antibody specific for PRTFDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRTFDC1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1), ELISA Kit
Full Product Name
Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit
Product Gene Name
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PRTFDC1. No significant cross-reactivity or interference between Human PRTFDC1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
19,983 Da
OMIM
AF226056 mRNA
3D Structure
ModBase 3D Structure for Q9NRG1
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PRTFDC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRTFDC1 purchase

MBS282589 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRTFDC1. The ELISA analytical biochemical technique of the MBS282589 kit is based on PRTFDC1 antibody-PRTFDC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRTFDC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRTFDC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRTFDC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphoribosyltransferase domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
phosphoribosyl transferase domain containing 1
NCBI Official Symbol
PRTFDC1
NCBI Official Synonym Symbols
HHGP
UniProt Protein Name
Phosphoribosyltransferase domain-containing protein 1
UniProt Gene Name
PRTFDC1
UniProt Synonym Gene Names
HHGP
NCBI Protein Information
phosphoribosyltransferase domain-containing protein 1

UniProt Comments for PRTFDC1

PRTFDC1: Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage. Belongs to the purine/pyrimidine phosphoribosyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: cytoplasm

Molecular Function: hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding; protein binding; protein homodimerization activity

Biological Process: GMP catabolic process; guanine salvage; purine ribonucleoside salvage
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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