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Growth Differentiation Factor 9 (GDF9) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2097272
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MBS2097272 is an active protein targeting GDF9. Also known as Active Growth Differentiation Factor 9 (GDF9). Reactive in Human. Host: E Coli. More details

$305 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Growth Differentiation Factor 9 (GDF9), Active Protein
Full Product Name
Active Growth Differentiation Factor 9 (GDF9)
Product Gene Name
GDF9 active protein
Host
E Coli
Species Reactivity
Human
Form/Format
Freeze-dried powder
Purity/Purification
> 90%
Molecular Weight
Predicted Molecular Weight: 16.8kDa
Accurate Molecular Weight: 18kDa
OMIM
AC004500 Genomic DNA
3D Structure
ModBase 3D Structure for O60383
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Gly320~Arg454
Isoelectric Point
7.1

Product Categories/Family for GDF9 active protein

Cytokine; Metabolic pathway; Developmental science; Bone metabolism
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for GDF9 active protein
Cell culture, Activity Assays

Activity Data of GDF9 active protein

GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFbeta) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner.
Human GDF9 Active Protein – Cat# MBS2097272

SDS-Page of GDF9 active protein

Human GDF9 Active Protein, SDS-Page – Cat# MBS2097272

Testing Data of GDF9 active protein

Human GDF9 Active Protein, Testing Data – Cat# MBS2097272

Other Notes

Small volumes of GDF9 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GDF9. It may not necessarily be applicable to this product.

NCBI GI #
572152931
NCBI GeneID
2661
NCBI Accession #
NP_001275753.1
UniProt Primary Accession #
O60383
UniProt Secondary Accession #
Q4VAW5
UniProt Related Accession #
O60383
GenBank Nucleotide #
NM_001288824.2
NCBI Official Full Name
growth/differentiation factor 9 isoform 2
NCBI Official Synonym Full Names
growth differentiation factor 9
NCBI Official Symbol
GDF9
UniProt Protein Name
Growth/differentiation factor 9
Protein Family
Growth/differentiation factor
UniProt Gene Name
GDF9
UniProt Synonym Gene Names
GDF-9
NCBI Protein Information
growth/differentiation factor 9

NCBI Summary for GDF9

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins. [provided by RefSeq, Jul 2016]
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UniProt Comments for GDF9

GDF9: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cell development; female gamete generation; negative regulation of cell growth; oocyte growth; positive regulation of cell proliferation; regulation of apoptosis; regulation of MAPK cascade; transforming growth factor beta receptor signaling pathway
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Research Articles on GDF9

1. Asiaticoside hindered the invasive growth of KFs by inhibiting the GDF-9/MAPK/Smad pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GDF9 active protein

 Reference Product  PubMed Publications
 BMPR2 active protein  >37 publications with GDF9 and BMPR2
 SMAD2 active protein  >25 publications with GDF9 and SMAD2
 BMPR1B active protein  >24 publications with GDF9 and BMPR1B
 HAS2 active protein  >22 publications with GDF9 and HAS2
 FSHR active protein  >21 publications with GDF9 and FSHR
 CYP19A1 active protein  >20 publications with GDF9 and CYP19A1
 LHCGR active protein  >12 publications with GDF9 and LHCGR
 TGFBR1 active protein  >10 publications with GDF9 and TGFBR1
 CYP17A1 active protein  >6 publications with GDF9 and CYP17A1
 KITLG active protein  >2 publications with GDF9 and KITLG

Pathways associated with GDF9 active protein

 Products by Pathway  Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram

Diseases associated with GDF9 active protein

 Disease Name  Pubmed Publications
 Infertility, Female Antibodies  >74 publications with GDF9 and Infertility, Female
 Ovarian Diseases Antibodies  >72 publications with GDF9 and Ovarian Diseases
 Polycystic Ovary Syndrome Antibodies  >18 publications with GDF9 and Polycystic Ovary Syndrome
 Infertility, Male Antibodies  >10 publications with GDF9 and Infertility, Male
 Congenital Abnormalities Antibodies  >7 publications with GDF9 and Congenital Abnormalities
 Necrosis Antibodies  >5 publications with GDF9 and Necrosis
 Urogenital Abnormalities Antibodies  >4 publications with GDF9 and Urogenital Abnormalities
 Kidney Diseases Antibodies  >4 publications with GDF9 and Kidney Diseases
 Disorders of Sex Development Antibodies  >4 publications with GDF9 and Disorders of Sex Development
 Cardiovascular Diseases Antibodies  >4 publications with GDF9 and Cardiovascular Diseases

Organs/Tissues associated with GDF9 active protein

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >19 publications with GDF9 and Testis
 Kidney Antibodies  >12 publications with GDF9 and Kidney
 Brain Antibodies  >11 publications with GDF9 and Brain
 Liver Antibodies  >6 publications with GDF9 and Liver
 Adrenal Gland Antibodies  >2 publications with GDF9 and Adrenal Gland
 Mammary Gland Antibodies  >1 publications with GDF9 and Mammary Gland
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