Achaete-Scute Homolog 1 (ASCL1) ELISA Kit for Rat

Name: Rat ASCL1 ELISA Kit; Achaete-Scute Homolog 1; MyBioSource
Brand: MyBioSource
SKU: MBS282734
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282734

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASCL1 in samples. An antibody specific for ASCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASCL1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Achaete-scute homolog 1 (ASCL1), ELISA Kit

Full Product Name

Rat Achaete-scute homolog 1 (ASCL1) ELISA Kit

Product Gene Name

ASCL1 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat ASCL1. No significant cross-reactivity or interference between Rat ASCL1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

24,972 Da

3D Structure

ModBase 3D Structure for P19359

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ASCL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASCL1 purchase

MBS282734 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Achaete-scute homolog 1 (ASCL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASCL1. The ELISA analytical biochemical technique of the MBS282734 kit is based on ASCL1 antibody-ASCL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASCL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASCL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ASCL1. It may not necessarily be applicable to this product.

NCBI GI #

11693148

NCBI GeneID

64186

NCBI Accession #

NP_071779.1

UniProt Primary Accession #

P19359

UniProt Related Accession #

P19359

GenBank Nucleotide #

NM_022384.1

NCBI Official Full Name

achaete-scute homolog 1

NCBI Official Synonym Full Names

achaete-scute family bHLH transcription factor 1

NCBI Official Symbol

Ascl1

NCBI Official Synonym Symbols

Mash1

UniProt Protein Name

Achaete-scute homolog 1

Protein Family

Achaete-scute

UniProt Gene Name

Ascl1

UniProt Synonym Gene Names

Ash1; Mash-1

NCBI Protein Information

achaete-scute homolog 1

NCBI Summary for ASCL1

plays a role in neurogenesis of the autonomic nervous system [RGD, Feb 2006]

UniProt Comments for ASCL1

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation.

Research Articles on ASCL1

1. miR-34a appears to play an important role in the process of liver fibrosis by targeting ACSL1 and may show promise as a therapeutic molecular target for hepatic fibrosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ASCL1 elisa kit

Products by Pathway	Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
SIDS Susceptibility Pathways antibodies	SIDS Susceptibility Pathways Diagram

Diseases associated with ASCL1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>109 publications with ASCL1 and Neoplasms
Lung Diseases Antibodies	>41 publications with ASCL1 and Lung Diseases
Brain Diseases Antibodies	>25 publications with ASCL1 and Brain Diseases
Disease Models, Animal Antibodies	>17 publications with ASCL1 and Disease Models, Animal
Prostatic Diseases Antibodies	>7 publications with ASCL1 and Prostatic Diseases
Neoplasms, Experimental Antibodies	>5 publications with ASCL1 and Neoplasms, Experimental
Hyperplasia Antibodies	>5 publications with ASCL1 and Hyperplasia
Liver Neoplasms Antibodies	>5 publications with ASCL1 and Liver Neoplasms
Drug Toxicity Antibodies	>4 publications with ASCL1 and Drug Toxicity
Nervous System Malformations Antibodies	>4 publications with ASCL1 and Nervous System Malformations

Organs/Tissues associated with ASCL1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>167 publications with ASCL1 and Brain
Embryonic Tissue Antibodies	>81 publications with ASCL1 and Embryonic Tissue
Uncharacterized Tissue Antibodies	>1 publications with ASCL1 and Uncharacterized Tissue