Pigment Epithelium-Derived Factor (PEDF) ELISA Kit for Human

Name: Human PEDF ELISA Kit; Pigment Epithelium-Derived Factor; MyBioSource
Brand: MyBioSource
SKU: MBS283319
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283319

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PEDF in samples. An antibody specific for PEDF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEDF present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pigment epithelium-derived factor (PEDF), ELISA Kit

Full Product Name

Human Pigment epithelium-derived factor (PEDF) ELISA Kit

Product Gene Name

PEDF elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PEDF. No significant cross-reactivity or interference between Human PEDF and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

46,312 Da

OMIM

gene 613982

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PEDF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEDF purchase

MBS283319 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pigment epithelium-derived factor (PEDF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PEDF. The ELISA analytical biochemical technique of the MBS283319 kit is based on PEDF antibody-PEDF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEDF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEDF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PEDF. It may not necessarily be applicable to this product.

NCBI GI #

39725934

NCBI GeneID

5176

NCBI Accession #

NP_002606.3

UniProt Secondary Accession #

Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092

UniProt Related Accession #

P36955

GenBank Nucleotide #

NM_002615.5

NCBI Official Full Name

pigment epithelium-derived factor

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

NCBI Official Symbol

SERPINF1

NCBI Official Synonym Symbols

OI6; OI12; PEDF; EPC-1; PIG35

UniProt Protein Name

Pigment epithelium-derived factor

UniProt Synonym Protein Names

Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

Protein Family

Pigment epithelium-derived factor

UniProt Gene Name

SERPINF1

UniProt Synonym Gene Names

PEDF; PEDF

UniProt Entry Name

PEDF_HUMAN

NCBI Protein Information

pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

NCBI Summary for PEDF

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

UniProt Comments for PEDF

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; multicellular organismal development; negative regulation of inflammatory response; response to arsenic; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi

Research Articles on PEDF

1. PEDF represents a novel intrinsic antioxidant of granulosa cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PEDF elisa kit

Reference Product	PubMed Publications
VEGFA elisa kit	>2 publications with PEDF and VEGFA
A2M elisa kit	>1 publications with PEDF and A2M

Diseases associated with PEDF elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>3 publications with PEDF and Nervous System Diseases
Brain Diseases Antibodies	>1 publications with PEDF and Brain Diseases
Proteinuria Antibodies	>1 publications with PEDF and Proteinuria
Urinary Bladder Neoplasms Antibodies	>1 publications with PEDF and Urinary Bladder Neoplasms
Lung Neoplasms Antibodies	>1 publications with PEDF and Lung Neoplasms
Cell Transformation, Neoplastic Antibodies	>1 publications with PEDF and Cell Transformation, Neoplastic
Weight Loss Antibodies	>1 publications with PEDF and Weight Loss

Organs/Tissues associated with PEDF elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>21 publications with PEDF and Bone
Nerve Antibodies	>19 publications with PEDF and Nerve
Eye Antibodies	>17 publications with PEDF and Eye
Connective Tissue Antibodies	>8 publications with PEDF and Connective Tissue
Blood Antibodies	>6 publications with PEDF and Blood
Skin Antibodies	>5 publications with PEDF and Skin
Adipose Tissue Antibodies	>5 publications with PEDF and Adipose Tissue
Brain Antibodies	>5 publications with PEDF and Brain
Embryonic Tissue Antibodies	>3 publications with PEDF and Embryonic Tissue
Lung Antibodies	>2 publications with PEDF and Lung