Lysozyme (LZM) ELISA Kit for Human

Lysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase, are a family of enzymes (EC 3.2.1.17) which damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of the polymorphonuclear neutrophils (PMN). Large amounts of lysozyme can be found in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LZM in samples. An antibody specific for LZM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLZM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LZM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LZM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of LZM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283820 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysozyme (LZM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LZM. The ELISA analytical biochemical technique of the MBS283820 kit is based on LZM antibody-LZM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LZM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LZM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).