Interleukin 33 (IL-33) ELISA Kit for Human

Name: Human IL-33 ELISA Kit; Interleukin 33; MyBioSource
Brand: MyBioSource
SKU: MBS283905
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283905

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL33 in samples. An antibody specific for IL33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL33 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin 33 (IL-33), ELISA Kit

Full Product Name

Human Interleukin 33 (IL-33) ELISA Kit

Product Gene Name

IL-33 elisa kit

Product Synonym Names

C9orf26; DKFZp586H0523; DVS27; NF-HEV; NFEHEV; RP11-575C20.2; DVS27-related protein|nuclear factor from high endothelial venules

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human IL33. No significant cross-reactivity or interference between Human IL33 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

16,250 Da

OMIM

AB024518 mRNA

3D Structure

ModBase 3D Structure for O95760

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IL-33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-33 purchase

MBS283905 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 33 (IL-33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-33. The ELISA analytical biochemical technique of the MBS283905 kit is based on IL-33 antibody-IL-33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-33. It may not necessarily be applicable to this product.

NCBI GI #

313851030

NCBI GeneID

90865

NCBI Accession #

NP_001186569.1

UniProt Primary Accession #

O95760

UniProt Secondary Accession #

Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4

UniProt Related Accession #

O95760

GenBank Nucleotide #

NM_001199640.1

NCBI Official Full Name

interleukin-33 isoform b

NCBI Official Synonym Full Names

interleukin 33

NCBI Official Symbol

IL33

NCBI Official Synonym Symbols

DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26

UniProt Protein Name

Interleukin-33

UniProt Synonym Protein Names

Interleukin-1 family member 11; IL-1F11

Protein Family

Interleukin

UniProt Gene Name

IL33

UniProt Synonym Gene Names

C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV

NCBI Protein Information

interleukin-33

NCBI Summary for IL-33

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

UniProt Comments for IL-33

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: chromosome; cytosol; extracellular region; extracellular space; nucleoplasm; transport vesicle

Molecular Function: cytokine activity; protein binding

Biological Process: defense response to virus; microglial cell activation involved in immune response; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; negative regulation of leukocyte migration; negative regulation of T-helper 1 type immune response; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of interleukin-5 production; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of type 2 immune response; protein deubiquitination; transcription, DNA-dependent; type 2 immune response

Research Articles on IL-33

1. IL-33 is closely interlinked in AD skins and keratinocytes. IL-33 plays an important role in the pathogenesis of immune inflammatory responses in AD, which might be a possible therapeutic target in the treatment of AD.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL-33 elisa kit

Reference Product	PubMed Publications
IL1RL1 elisa kit	>161 publications with IL-33 and IL1RL1
TSLP elisa kit	>19 publications with IL-33 and TSLP
CASP1 elisa kit	>17 publications with IL-33 and CASP1
IL18 elisa kit	>17 publications with IL-33 and IL18
IL25 elisa kit	>14 publications with IL-33 and IL25
IL18R1 elisa kit	>7 publications with IL-33 and IL18R1
VEGFA elisa kit	>3 publications with IL-33 and VEGFA
IL1B elisa kit	>2 publications with IL-33 and IL1B
CSF2 elisa kit	>1 publications with IL-33 and CSF2

Pathways associated with IL-33 elisa kit

Products by Pathway	Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram

Diseases associated with IL-33 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>191 publications with IL-33 and Inflammation
Asthma Antibodies	>91 publications with IL-33 and Asthma
Hypersensitivity, Immediate Antibodies	>87 publications with IL-33 and Hypersensitivity, Immediate
Lung Diseases Antibodies	>86 publications with IL-33 and Lung Diseases
Necrosis Antibodies	>55 publications with IL-33 and Necrosis
Cardiovascular Diseases Antibodies	>51 publications with IL-33 and Cardiovascular Diseases
Heart Diseases Antibodies	>30 publications with IL-33 and Heart Diseases
Fibrosis Antibodies	>22 publications with IL-33 and Fibrosis
Disease Models, Animal Antibodies	>19 publications with IL-33 and Disease Models, Animal
Dermatitis, Atopic Antibodies	>18 publications with IL-33 and Dermatitis, Atopic

Organs/Tissues associated with IL-33 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>55 publications with IL-33 and Lung
Skin Antibodies	>43 publications with IL-33 and Skin
Heart Antibodies	>43 publications with IL-33 and Heart
Vascular Antibodies	>33 publications with IL-33 and Vascular
Muscle Antibodies	>19 publications with IL-33 and Muscle
Intestine Antibodies	>17 publications with IL-33 and Intestine
Liver Antibodies	>16 publications with IL-33 and Liver
Brain Antibodies	>12 publications with IL-33 and Brain
Eye Antibodies	>9 publications with IL-33 and Eye
Connective Tissue Antibodies	>8 publications with IL-33 and Connective Tissue