HEXIM1 ELISA Kit for Rat

Name: Rat HEXIM1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS284026
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284026

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HEXIM1 in samples. An antibody specific for HEXIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHEXIM1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

HEXIM1, ELISA Kit

Full Product Name

Rat Protein HEXIM1 (HEXIM1) ELISA Kit

Product Gene Name

HEXIM1 elisa kit

Product Synonym Names

CLP1; EDG1; FLJ13562; HIS1; MAQ1; HMBA-inducible|hexamethylene bisacetamide-inducible protein|hexamethylene-bis-acetamide-inducible transcript 1|hexamthylene bis-acetamide inducible 1|menage a quatr

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat HEXIM1. No significant cross-reactivity or interference between Rat HEXIM1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

40,317 Da

3D Structure

ModBase 3D Structure for Q5M9G1

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HEXIM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEXIM1 purchase

MBS284026 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HEXIM1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HEXIM1. The ELISA analytical biochemical technique of the MBS284026 kit is based on HEXIM1 antibody-HEXIM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HEXIM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HEXIM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HEXIM1. It may not necessarily be applicable to this product.

NCBI GI #

68342005

NCBI GeneID

498008

NCBI Accession #

NP_001020307.1

UniProt Primary Accession #

Q5M9G1

UniProt Related Accession #

Q5M9G1

GenBank Nucleotide #

NM_001025136.1

NCBI Official Full Name

protein HEXIM1

NCBI Official Synonym Full Names

hexamethylene bis-acetamide inducible 1

NCBI Official Symbol

Hexim1

NCBI Official Synonym Symbols

Clp1; Clp-1

UniProt Protein Name

Protein HEXIM1

UniProt Synonym Protein Names

Cardiac lineage protein 1

Protein Family

Protein

UniProt Gene Name

Hexim1

UniProt Synonym Gene Names

Clp1

NCBI Protein Information

protein HEXIM1

UniProt Comments for HEXIM1

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 10q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein serine/threonine kinase inhibitor activity; snRNA binding

Biological Process: activation of innate immune response; heart development; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with HEXIM1 elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>9 publications with HEXIM1 and Heart Diseases
Cardiomyopathies Antibodies	>3 publications with HEXIM1 and Cardiomyopathies
Ovarian Neoplasms Antibodies	>2 publications with HEXIM1 and Ovarian Neoplasms
Skin Diseases Antibodies	>2 publications with HEXIM1 and Skin Diseases
Fibrosis Antibodies	>2 publications with HEXIM1 and Fibrosis
Hematologic Diseases Antibodies	>2 publications with HEXIM1 and Hematologic Diseases
Drug Toxicity Antibodies	>1 publications with HEXIM1 and Drug Toxicity
Inflammation Antibodies	>1 publications with HEXIM1 and Inflammation
Thrombocytopenia Antibodies	>1 publications with HEXIM1 and Thrombocytopenia
Myocardial Ischemia Antibodies	>1 publications with HEXIM1 and Myocardial Ischemia