Interleukin-36 Gamma (IL1F9) ELISA Kit for Human

IL1F9 encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2, and is specifically inhibited by interleukin 1 family, member 5 . Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL1F9 in samples. An antibody specific for IL1F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL1F9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of IL1F9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285236 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-36 gamma (IL1F9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL1F9. The ELISA analytical biochemical technique of the MBS285236 kit is based on IL1F9 antibody-IL1F9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1F9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1F9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).