Matrix Metalloproteinase 8/Neutrophil Collagenase (MMP8) ELISA Kit for Human

Name: Human MMP8 ELISA Kit; Matrix Metalloproteinase 8/Neutrophil Collagenase; MyBioSource
Brand: MyBioSource
SKU: MBS285250
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285250

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MMP8 in samples. An antibody specific for MMP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP8 present is bound by the… More details

$370 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Matrix metalloproteinase 8/Neutrophil collagenase (MMP8), ELISA Kit

Full Product Name

Human Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit

Product Gene Name

MMP8 elisa kit

Product Synonym Names

CLG1, HNC, PMNL-CL, PMNL collagenase|matrix metalloproteinase 8|matrix metalloproteinase 8 (neutrophil collagenase)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MMP8. No significant cross-reactivity or interference between Human MMP8 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

53,412 Da

OMIM

J05556 mRNA

3D Structure

ModBase 3D Structure for P22894

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MMP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP8 purchase

MBS285250 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix metalloproteinase 8/Neutrophil collagenase (MMP8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP8. The ELISA analytical biochemical technique of the MBS285250 kit is based on MMP8 antibody-MMP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.

NCBI GI #

751247063

NCBI GeneID

4317

NCBI Accession #

NP_001291370.1

UniProt Primary Accession #

P22894

UniProt Secondary Accession #

Q45F99

UniProt Related Accession #

P22894

GenBank Nucleotide #

NM_001304441.1

NCBI Official Full Name

neutrophil collagenase isoform 2

NCBI Official Synonym Full Names

matrix metallopeptidase 8

NCBI Official Symbol

MMP8

NCBI Official Synonym Symbols

HNC; CLG1; MMP-8; PMNL-CL

UniProt Protein Name

Neutrophil collagenase

UniProt Synonym Protein Names

Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL

Protein Family

Neutrophil collagenase

UniProt Gene Name

MMP8

UniProt Synonym Gene Names

CLG1; MMP-8; PMNL-CL

NCBI Protein Information

neutrophil collagenase

NCBI Summary for MMP8

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

UniProt Comments for MMP8

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.34; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; serine-type endopeptidase activity; zinc ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; neutrophil degranulation; proteolysis

Research Articles on MMP8

1. The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MMP8 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with MMP8 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>97 publications with MMP8 and Inflammation
Cardiovascular Diseases Antibodies	>50 publications with MMP8 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>31 publications with MMP8 and Disease Models, Animal
Fibrosis Antibodies	>31 publications with MMP8 and Fibrosis
Necrosis Antibodies	>21 publications with MMP8 and Necrosis
Lung Diseases Antibodies	>21 publications with MMP8 and Lung Diseases
Atherosclerosis Antibodies	>18 publications with MMP8 and Atherosclerosis
Hemorrhage Antibodies	>17 publications with MMP8 and Hemorrhage
Heart Diseases Antibodies	>17 publications with MMP8 and Heart Diseases
Wounds and Injuries Antibodies	>15 publications with MMP8 and Wounds and Injuries

Organs/Tissues associated with MMP8 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>106 publications with MMP8 and Blood
Connective Tissue Antibodies	>18 publications with MMP8 and Connective Tissue
Skin Antibodies	>15 publications with MMP8 and Skin
Liver Antibodies	>13 publications with MMP8 and Liver
Bone Marrow Antibodies	>9 publications with MMP8 and Bone Marrow
Thymus Antibodies	>1 publications with MMP8 and Thymus