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Adenosine Deaminase (ADA) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285684

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ADA in samples. An antibody specific for ADA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADA present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Adenosine deaminase (ADA), ELISA Kit
Full Product Name
Rat Adenosine deaminase (ADA) ELISA Kit
Product Gene Name
ADA elisa kit
Product Synonym Names
Adenosine aminohydrolase
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ADA. No significant cross-reactivity or interference between Rat ADA and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
39,899 Da
3D Structure
ModBase 3D Structure for Q920P6
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ADA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADA purchase

MBS285684 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine deaminase (ADA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADA. The ELISA analytical biochemical technique of the MBS285684 kit is based on ADA antibody-ADA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADA. It may not necessarily be applicable to this product.

NCBI GI #
18426812
NCBI GeneID
24165
NCBI Accession #
NP_569083.1
UniProt Primary Accession #
Q920P6
UniProt Related Accession #
Q920P6
GenBank Nucleotide #
NM_130399.2
NCBI Official Full Name
adenosine deaminase
NCBI Official Synonym Full Names
adenosine deaminase
NCBI Official Symbol
Ada
UniProt Protein Name
Adenosine deaminase
UniProt Synonym Protein Names
Adenosine aminohydrolase
Protein Family
Bifunctional transcriptional activator/DNA repair enzyme
UniProt Gene Name
Ada
NCBI Protein Information
adenosine deaminase

NCBI Summary for ADA

catalyzes the conversion of adenosine to inosine in adenosine metabolism; may act as a neuormodulator in sleep-wake regulation [RGD, Feb 2006]

UniProt Comments for ADA

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: EC 3.5.4.4; Hydrolase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 3q42

Cellular Component: cell junction; cell soma; cell surface; cytoplasm; cytosol; dendrite cytoplasm; external side of plasma membrane; extracellular space; lysosome; membrane

Molecular Function: adenosine deaminase activity; purine nucleoside binding; zinc ion binding

Biological Process: adenosine catabolic process; adenosine metabolic process; aging; alveolus development; cell adhesion; dATP catabolic process; deoxyadenosine catabolic process; embryonic gut development; germinal center B cell differentiation; histamine secretion; hypoxanthine biosynthetic process; hypoxanthine salvage; in utero embryonic development; inosine biosynthetic process; liver development; lung development; negative regulation of adenosine receptor signaling pathway; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of inflammatory response; negative regulation of leukocyte migration; negative regulation of mature B cell apoptosis; Peyer's patch development; placenta development; positive regulation of alpha-beta T cell differentiation; positive regulation of B cell proliferation; positive regulation of calcium-mediated signaling; positive regulation of germinal center formation; positive regulation of heart rate; positive regulation of smooth muscle contraction; positive regulation of T cell activation; positive regulation of T cell differentiation; positive regulation of T cell differentiation in thymus; positive regulation of T cell receptor signaling pathway; purine nucleotide salvage; purine ribonucleoside monophosphate biosynthetic process; regulation of cell-cell adhesion mediated by integrin; regulation of circadian sleep/wake cycle, sleep; regulation of T cell differentiation; response to drug; response to hydrogen peroxide; response to hypoxia; response to morphine; response to vitamin E; T cell activation; trophectodermal cell differentiation; xanthine biosynthetic process
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Research Articles on ADA

1. Quercetin decreases NTPDase, 5'-nucleotidase and adenosine deaminase activities in blood platelets of hypothyroid rats, and improves the oxidative profile to reduce platelet aggregation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADA elisa kit

 Reference Product  PubMed Publications
 Dpp4 elisa kit  >21 publications with ADA and Dpp4
 Dck elisa kit  >10 publications with ADA and Dck
 Adk elisa kit  >3 publications with ADA and Adk
 Nt5e elisa kit  >2 publications with ADA and Nt5e

Pathways associated with ADA elisa kit

 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Salvage Pathway antibodies  Purine Salvage Pathway Diagram

Diseases associated with ADA elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >448 publications with ADA and Nervous System Diseases
 Brain Diseases Antibodies  >220 publications with ADA and Brain Diseases
 Heart Diseases Antibodies  >199 publications with ADA and Heart Diseases
 Inflammation Antibodies  >198 publications with ADA and Inflammation
 Necrosis Antibodies  >188 publications with ADA and Necrosis
 Hypertension Antibodies  >165 publications with ADA and Hypertension
 Disease Models, Animal Antibodies  >131 publications with ADA and Disease Models, Animal
 Pain Antibodies  >109 publications with ADA and Pain
 Liver Diseases Antibodies  >97 publications with ADA and Liver Diseases
 Kidney Diseases Antibodies  >79 publications with ADA and Kidney Diseases

Organs/Tissues associated with ADA elisa kit

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >217 publications with ADA and Heart
 Lung Antibodies  >184 publications with ADA and Lung
 Thymus Antibodies  >130 publications with ADA and Thymus
 Embryonic Tissue Antibodies  >36 publications with ADA and Embryonic Tissue
 Small Intestine Antibodies  >35 publications with ADA and Small Intestine
 Placenta Antibodies  >27 publications with ADA and Placenta
 Inner Ear Antibodies  >1 publications with ADA and Inner Ear
 Uncharacterized Tissue Antibodies  >1 publications with ADA and Uncharacterized Tissue
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