Human EIF2AK3 ELISA Kit – Cat# MBS287695

Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3 (EIF2AK3) ELISA Kit for Human

Name: Human EIF2AK3 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS287695
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS287695

This assay employs a two-site sandwich ELISA to quantitate EIF2AK3 in samples. An antibody specific for EIF2AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF2AK3 present is bound by the immobilized… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), ELISA Kit

Full Product Name

Human Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) ELISA Kit

Product Gene Name

EIF2AK3 elisa kit

Product Synonym Names

DKFZp781H1925; HRI; PEK; PERK; WRS; eukaryotic translation initiation factor 2 alpha kinase 3

Species Reactivity

Human

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other Biological Fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human EIF2AK3. No significant cross-reactivity or interference between Human EIF2AK3 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

125,216 Da

OMIM

AF110146 mRNA

3D Structure

ModBase 3D Structure for Q9NZJ5

Detection Wavelength

450 nm

Intra-assay Precision (Precision within an assay)

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

CV (%)

= SD/meanX100

Intra-Assay

Inter-Assay

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of EIF2AK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF2AK3 purchase

MBS287695 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EIF2AK3. The ELISA analytical biochemical technique of the MBS287695 kit is based on EIF2AK3 antibody-EIF2AK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2AK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2AK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2AK3. It may not necessarily be applicable to this product.

NCBI GI #

134304838

NCBI GeneID

9451

NCBI Accession #

NP_004827.4

UniProt Primary Accession #

Q9NZJ5

UniProt Secondary Accession #

O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9

UniProt Related Accession #

Q9NZJ5

GenBank Nucleotide #

NM_004836.6

NCBI Official Full Name

eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 2 alpha kinase 3

NCBI Official Symbol

EIF2AK3

NCBI Official Synonym Symbols

PEK; WRS; PERK

UniProt Protein Name

Eukaryotic translation initiation factor 2-alpha kinase 3

UniProt Synonym Protein Names

PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

Protein Family

Eukaryotic translation initiation factor 2-alpha kinase

UniProt Gene Name

EIF2AK3

UniProt Synonym Gene Names

PEK; PERK; HsPEK

NCBI Protein Information

eukaryotic translation initiation factor 2-alpha kinase 3

NCBI Summary for EIF2AK3

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

UniProt Comments for EIF2AK3

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Research Articles on EIF2AK3

1. miR-204 Targets PERK and Regulates UPR Signaling and beta-Cell Apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EIF2AK3 elisa kit

Reference Product	PubMed Publications
HSPA5 elisa kit	>2 publications with EIF2AK3 and HSPA5
EIF2S1 elisa kit	>1 publications with EIF2AK3 and EIF2S1
DNAJC3 elisa kit	>1 publications with EIF2AK3 and DNAJC3

Pathways associated with EIF2AK3 elisa kit

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Diseases associated with EIF2AK3 elisa kit

Disease Name	Pubmed Publications
Wolcott-Rallison syndrome Antibodies	>28 publications with EIF2AK3 and Wolcott-Rallison syndrome
Nervous System Diseases Antibodies	>6 publications with EIF2AK3 and Nervous System Diseases
Liver Diseases Antibodies	>5 publications with EIF2AK3 and Liver Diseases
Brain Diseases Antibodies	>5 publications with EIF2AK3 and Brain Diseases
Supranuclear Palsy, Progressive Antibodies	>3 publications with EIF2AK3 and Supranuclear Palsy, Progressive
Drug Toxicity Antibodies	>2 publications with EIF2AK3 and Drug Toxicity
Renal Insufficiency Antibodies	>2 publications with EIF2AK3 and Renal Insufficiency
Hepatitis Antibodies	>2 publications with EIF2AK3 and Hepatitis
Atrophy Antibodies	>1 publications with EIF2AK3 and Atrophy
Cognition Disorders Antibodies	>1 publications with EIF2AK3 and Cognition Disorders

Organs/Tissues associated with EIF2AK3 elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>16 publications with EIF2AK3 and Pancreas
Bone Antibodies	>13 publications with EIF2AK3 and Bone
Liver Antibodies	>11 publications with EIF2AK3 and Liver
Brain Antibodies	>8 publications with EIF2AK3 and Brain
Blood Antibodies	>5 publications with EIF2AK3 and Blood
Heart Antibodies	>2 publications with EIF2AK3 and Heart
Intestine Antibodies	>1 publications with EIF2AK3 and Intestine
Skin Antibodies	>1 publications with EIF2AK3 and Skin
Muscle Antibodies	>1 publications with EIF2AK3 and Muscle
Thyroid Antibodies	>1 publications with EIF2AK3 and Thyroid