Cyclin-Dependent Kinase 12 (CDK12) ELISA Kit for Mouse

CDK12: a Ser/Thr protein kinase of the CMGC group and CDK family. Phosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Interacts with CCNL1 and CCNL2. Colocalized with nuclear speckles throughout interphase. Has a highly phosphorylated arginine/serine-rich (RS) domain N-terminal to the kinase domain. Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; Protein kinase, CMGC; RNA splicing; EC 2.7.11.22; Motility/polarity/chemotaxis; EC 2.7.11.23; CMGC group; CDK family; CDK/CRK7 subfamily; CRK7 subfamily

Cellular Component: nuclear cyclin-dependent protein kinase holoenzyme complex; nuclear speck; nucleolus; nucleus

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity; transferase activity

Biological Process: mRNA processing; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of MAP kinase activity; regulation of RNA splicing; RNA splicing