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A Disintegrin and Metalloprotease 10 (ADAM10) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS029062

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of ADAM10 (hereafter termed "analyte") in undiluted… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
A Disintegrin and Metalloprotease 10 (ADAM10), ELISA Kit
Full Product Name
Mouse A Disintegrin and Metalloprotease 10 ELISA Kit
Product Gene Name
ADAM10 elisa kit
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Detection Range
31.2pg/ml-1000pg/ml
Sensitivity
5.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Molecular Weight
84,142 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (58888510..59042177, complement). Location: 15q22
OMIM
602192
3D Structure
ModBase 3D Structure for O14672

Other Notes

Small volumes of ADAM10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAM10 purchase

MBS029062 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 10 (ADAM10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM10. The ELISA analytical biochemical technique of the MBS029062 kit is based on ADAM10 antibody-ADAM10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.

NCBI GI #
2393947
NCBI GeneID
102
NCBI Accession #
AAC51766.1
UniProt Primary Accession #
O14672
UniProt Secondary Accession #
Q10742; Q92650
UniProt Related Accession #
O14672
NCBI Official Full Name
ADAM10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10
NCBI Official Synonym Symbols
kuz; AD10; MADM; CD156c; HsT18717
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Synonym Protein Names
CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease
Protein Family
Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name
ADAM10
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10
UniProt Entry Name
ADA10_HUMAN
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10

NCBI Summary for ADAM10

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

UniProt Comments for ADAM10

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Cell adhesion; Membrane protein, integral; Protease; Cell surface; Motility/polarity/chemotaxis; EC 3.4.24.81; Vesicle

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; cell surface; focal adhesion; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; in utero embryonic development; membrane protein ectodomain proteolysis; constitutive protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; cell-cell signaling; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
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Research Articles on ADAM10

1. MiR-144 and Mir-451 negatively regulator the ADAM10 protein expression and may contribute to Alzheimer disease pathogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADAM10 elisa kit

 Reference Product  PubMed Publications
 ADAM17 elisa kit  >187 publications with ADAM10 and ADAM17
 EGFR elisa kit  >25 publications with ADAM10 and EGFR
 ADAM12 elisa kit  >24 publications with ADAM10 and ADAM12
 CTNNB1 elisa kit  >13 publications with ADAM10 and CTNNB1
 NOTCH1 elisa kit  >12 publications with ADAM10 and NOTCH1
 CDH2 elisa kit  >5 publications with ADAM10 and CDH2
 DLL1 elisa kit  >2 publications with ADAM10 and DLL1

Pathways associated with ADAM10 elisa kit

 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway antibodies  Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram

Diseases associated with ADAM10 elisa kit

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >179 publications with ADAM10 and Necrosis
 Neoplasms Antibodies  >118 publications with ADAM10 and Neoplasms
 Brain Diseases Antibodies  >101 publications with ADAM10 and Brain Diseases
 Inflammation Antibodies  >45 publications with ADAM10 and Inflammation
 Skin Diseases Antibodies  >27 publications with ADAM10 and Skin Diseases
 Kidney Diseases Antibodies  >17 publications with ADAM10 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >16 publications with ADAM10 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >16 publications with ADAM10 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >15 publications with ADAM10 and Neoplasm Metastasis
 Vascular Diseases Antibodies  >15 publications with ADAM10 and Vascular Diseases

Organs/Tissues associated with ADAM10 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >129 publications with ADAM10 and Brain
 Blood Antibodies  >88 publications with ADAM10 and Blood
 Vascular Antibodies  >40 publications with ADAM10 and Vascular
 Kidney Antibodies  >36 publications with ADAM10 and Kidney
 Embryonic Tissue Antibodies  >32 publications with ADAM10 and Embryonic Tissue
 Muscle Antibodies  >25 publications with ADAM10 and Muscle
 Nerve Antibodies  >22 publications with ADAM10 and Nerve
 Skin Antibodies  >20 publications with ADAM10 and Skin
 Bone Antibodies  >18 publications with ADAM10 and Bone
 Lung Antibodies  >17 publications with ADAM10 and Lung
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